中毒性休克综合征毒素1中组氨酸和酪氨酸残基的化学修饰对该毒素血清学活性和促有丝分裂活性的影响。
Effect of chemical modification of histidine and tyrosine residues in toxic shock syndrome toxin 1 on the serologic and mitogenic activities of the toxin.
作者信息
Kokan-Moore N P, Bergdoll M S
机构信息
Department of Food Microbiology and Toxicology, University of Wisconsin-Madison 53706.
出版信息
Infect Immun. 1989 Jul;57(7):1901-5. doi: 10.1128/iai.57.7.1901-1905.1989.
Abstract
Modification of three or four of the five histidine residues in the toxic shock syndrome toxin 1 (TSST-1) with diethylpyrocarbonate did not inhibit the precipitin reaction of the modified TSST-1 with polyvalent antisera to the toxin. Monoclonal antibody 7T did not react with the modified TSST-1, but monoclonal antibody 8T did react with the toxin. Up to 50% of the mitogenic reaction of TSST-1 was inhibited by the histidine modification. Modification of one or two of the nine tyrosine residues in TSST-1 did not inhibit the precipitin reaction with polyclonal antisera to the toxin but did inhibit 85% of the mitogenic reaction.
摘要
用焦碳酸二乙酯修饰中毒性休克综合征毒素1（TSST-1）的五个组氨酸残基中的三或四个，并不抑制修饰后的TSST-1与该毒素的多价抗血清的沉淀反应。单克隆抗体7T不与修饰后的TSST-1反应，但单克隆抗体8T确实与该毒素反应。组氨酸修饰抑制了高达50%的TSST-1促有丝分裂反应。修饰TSST-1的九个酪氨酸残基中的一或两个，并不抑制与该毒素的多克隆抗血清的沉淀反应，但确实抑制了85%的促有丝分裂反应。
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