Buermans H P J, den Dunnen J T
Leiden Genome Technology Center, Leiden University Medical Center, Postbus 9600, 2300 RC Leiden, The Netherlands.
Biochim Biophys Acta. 2014 Oct;1842(10):1932-1941. doi: 10.1016/j.bbadis.2014.06.015. Epub 2014 Jul 1.
Impressive progress has been made in the field of Next Generation Sequencing (NGS). Through advancements in the fields of molecular biology and technical engineering, parallelization of the sequencing reaction has profoundly increased the total number of produced sequence reads per run. Current sequencing platforms allow for a previously unprecedented view into complex mixtures of RNA and DNA samples. NGS is currently evolving into a molecular microscope finding its way into virtually every fields of biomedical research. In this chapter we review the technical background of the different commercially available NGS platforms with respect to template generation and the sequencing reaction and take a small step towards what the upcoming NGS technologies will bring. We close with an overview of different implementations of NGS into biomedical research. This article is part of a Special Issue entitled: From Genome to Function.
下一代测序（NGS）领域已取得了令人瞩目的进展。通过分子生物学和技术工程领域的进步，测序反应的并行化极大地增加了每次运行产生的序列读数总数。当前的测序平台能够以前所未有的视角观察RNA和DNA样本的复杂混合物。NGS目前正在演变成一种分子显微镜，几乎进入了生物医学研究的各个领域。在本章中，我们回顾了不同商用NGS平台在模板生成和测序反应方面的技术背景，并对即将出现的NGS技术将带来的变化迈出了一小步。我们最后概述了NGS在生物医学研究中的不同应用。本文是名为《从基因组到功能》的特刊的一部分。
