Schweitzer Matthias, Friedrich Andrea Marianne, Dennig Alexander, Berg Gabriele, Müller Bogotá Christina Andrea
Institute of Environmental Biotechnology, Graz University of Technology, Petersgasse 12/I, 8010 Graz, Austria.
Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12/I, 8010 Graz, Austria.
FEMS Microbiol Lett. 2025 Jan 10;372. doi: 10.1093/femsle/fnaf004.
Terminal olefins are important platform chemicals, drop-in compatible hydrocarbons and also play an important role as biocontrol agents of plant pathogens. Currently, 1-alkenes are derived from petroleum, although microbial biosynthetic routes are known. Jeotgalicoccus sp. ATCC 8456 produces 1-alkenes via the fatty acid decarboxylase OleTJE. UndA and UndB are recently identified non-heme iron oxidases converting medium-chain fatty acids into terminal alkenes. Our knowledge about the diversity and natural function of OleTJE, UndA, and UndB homologs is scarce. We applied a combined screening strategy-solid-phase microextraction coupled with gas chromatography-mass spectrometry (SPME GC-MS) and polymerase chain reaction (PCR)-based amplification-to survey an environmental strain collection for microbial 1-alkene producers and their corresponding enzymes. Our results reinforce the high level of conservation of UndA and UndB genes across the genus Pseudomonas. In vivo production of defined 1-alkenes (C9-C13; C15; C19) was directed by targeted feeding of fatty acids. Lauric acid feeding enabled 1-undecene production to a concentration of 3.05 mg l-1 in Jeotgalicoccus sp. ATCC 8456 and enhanced its production by 105% in Pseudomonas putida 1T1 (1.10 mg l-1). Besides, whole genome sequencing of Jeotgalicoccus sp. ATCC 8456 enabled reconstruction of the 1-alkene biosynthetic pathway. These results advance our understanding of microbial 1-alkene synthesis and the underlying genetic basis.
末端烯烃是重要的平台化学品、可直接替代的碳氢化合物，并且在作为植物病原体的生物防治剂方面也发挥着重要作用。目前，1-烯烃来源于石油，尽管已知微生物生物合成途径。嗜盐放线菌属菌株ATCC 8456通过脂肪酸脱羧酶OleTJE产生1-烯烃。UndA和UndB是最近鉴定出的非血红素铁氧化酶，可将中链脂肪酸转化为末端烯烃。我们对OleTJE、UndA和UndB同源物的多样性和天然功能的了解很少。我们应用了一种联合筛选策略——固相微萃取结合气相色谱-质谱联用（SPME GC-MS）和基于聚合酶链反应（PCR）的扩增——来调查一组环境菌株，以寻找微生物1-烯烃生产者及其相应的酶。我们的结果强化了假单胞菌属中UndA和UndB基因的高度保守性。通过有针对性地投喂脂肪酸，可定向体内合成特定的1-烯烃（C9-C13；C15；C19）。投喂月桂酸可使嗜盐放线菌属菌株ATCC 8456中1-十一碳烯的产量达到3.05 mg l-1，并使恶臭假单胞菌1T1中的产量提高105%（达到1.10 mg l-1）。此外，嗜盐放线菌属菌株ATCC 8456的全基因组测序使得1-烯烃生物合成途径得以重建。这些结果推进了我们对微生物1-烯烃合成及其潜在遗传基础的理解。
