Mertens P P, Crook N E, Rubinstein R, Pedley S, Payne C C
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, U.K.
J Gen Virol. 1989 Jan;70 ( Pt 1):173-85. doi: 10.1099/0022-1317-70-1-173.
Serological analyses of several different cytoplasmic polyhedrosis viruses (CPVs), including two type 1 CPVs from Bombyx mori, type 1 CPV from Dendrolimus spectabilis, type 12 CPV from Autographa gamma, type 2 CPV from Inachis io, type 5 CPV from Orgyia pseudotsugata and type 5 CPV from Heliothis armigera, demonstrated a close correlation between the antigenic properties of the polyhedrin or virus particle structural proteins and the genomic dsRNA electropherotypes. The dsRNAs of these viruses were analysed by electrophoresis in 3% and 10% polyacrylamide gels with a discontinuous Tris-HCl/Tris-glycine buffer system or by 1% agarose gel electrophoresis using a continuous Tris-acetate-EDTA buffer system. Electrophoretic analysis in agarose gels was found to be the most suitable for the classification of CPV isolates into electropherotypes, and the results obtained showed a close correlation with the observed antigenic relationships between different virus isolates. However, electrophoretic analysis in 10% polyacrylamide gels was most sensitive for the detection of intra-type variation and the presence of mixed virus isolates.
对几种不同的细胞质多角体病毒（CPV）进行血清学分析，包括来自家蚕的两种1型CPV、来自马尾松毛虫的1型CPV、来自粉纹夜蛾的12型CPV、来自眼蝶的2型CPV、来自云杉毒蛾的5型CPV和来自棉铃虫的5型CPV，结果表明多角体蛋白或病毒粒子结构蛋白的抗原特性与基因组双链RNA电泳图谱之间存在密切相关性。这些病毒的双链RNA通过在含不连续Tris-HCl/Tris-甘氨酸缓冲系统的3%和10%聚丙烯酰胺凝胶中进行电泳分析，或使用连续Tris-乙酸-EDTA缓冲系统通过1%琼脂糖凝胶电泳进行分析。发现琼脂糖凝胶电泳最适合将CPV分离株分类为电泳图谱类型，所得结果与不同病毒分离株之间观察到的抗原关系密切相关。然而，10%聚丙烯酰胺凝胶电泳对检测型内变异和混合病毒分离株的存在最为敏感。
