Payne C C, Rivers C F
J Gen Virol. 1976 Oct;33(1):71-85. doi: 10.1099/0022-1317-33-1-71.
The RNA genome segments of thirty-three isolates of cytoplasmic polyhedrosis viruses (CPVs) were examined by polyacrylamide gel electrophoresis. Major differences were observed in the gel profiles of the RNA segments from many of the viruses; differences which were reinforced by polyacrylamide gel electrophoresis of the virus structural proteins. As a result of these studies, a provisional classification scheme for CPVs is proposed, where viruses with similar RNA gel profiles are included within the same 'type', while isolates differing in the molecular weights of most, or all of the RNA segments are assigned to different types. Using this system, eleven distinct CPV types were recognized. All eleven CPV types, like reoviruses, probably contain ten segments of RNA with a toatl mol. wt. of approx. 15 X 10(6).
通过聚丙烯酰胺凝胶电泳对33株细胞质多角体病毒(CPV)的RNA基因组片段进行了检测。在许多病毒的RNA片段的凝胶图谱中观察到了主要差异;这些差异通过病毒结构蛋白的聚丙烯酰胺凝胶电泳得到了加强。作为这些研究的结果,提出了一种CPV的临时分类方案,其中具有相似RNA凝胶图谱的病毒被归入同一“类型”,而大多数或所有RNA片段分子量不同的分离株则被归为不同类型。使用该系统,识别出了11种不同的CPV类型。所有11种CPV类型,像呼肠孤病毒一样,可能都含有10个RNA片段,总分子量约为15×10⁶。