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脐带血单位相邻片段的质量控制:SFGM-TC的建议

Quality controls on cord blood unit contiguous segments: recommendation of the SFGM-TC.

作者信息

De Vos J, Birebent B, Faucher C, Giet O, Hicheri Y, Lemarie C, Baudoux E, Boulanger F, Garnier F, Larghero J, Pouthier F, Thibert J-B, Yakoub-Agha I

机构信息

Unité de thérapie cellulaire, CHU de Montpellier, 80, avenue Fliche, 34295 Montpellier cedex 5, France.

UITC, EFS Île-de-France, 5, rue Gustave-Eiffel, 94017 Créteil cedex, France.

出版信息

Pathol Biol (Paris). 2014 Aug;62(4):218-20. doi: 10.1016/j.patbio.2014.05.005. Epub 2014 Jul 1.

DOI:10.1016/j.patbio.2014.05.005
PMID:24996845
Abstract

In the attempt to harmonize clinical practices between different French transplantation centers, the French Society of Bone Marrow Transplantation and Cell Therapies (SFGM-TC) set up its fourth annual series of workshops which brought together practitioners from all of its member centers. These workshops took place in September 2013 in Lille. Literature and intra-laboratories studies suggest that attached segment is representative of cord blood unit (CBU). Nevertheless, some discrepancies have been observed when analyzing large data registries. To address these issues, we have listed recommendations to increase the standardization of segment processing and quality control (QC), information on units of measurement and specifications and action to be taken in case of out of specifications QC results on segment.

摘要

为协调法国不同移植中心之间的临床实践,法国骨髓移植与细胞治疗协会(SFGM-TC)举办了第四届年度系列研讨会,来自其所有成员中心的从业者齐聚一堂。这些研讨会于2013年9月在里尔举行。文献和实验室内部研究表明,附着片段代表脐带血单位(CBU)。然而,在分析大型数据登记处时发现了一些差异。为解决这些问题,我们列出了一些建议,以提高片段处理和质量控制(QC)的标准化、测量单位和规格信息,以及在片段QC结果不符合规格时应采取的行动。

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引用本文的文献

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Enumerating regulatory T cells in cryopreserved umbilical cord blood samples using FOXP3 methylation specific quantitative PCR.采用 FOXP3 甲基化特异性定量 PCR 技术对冷冻保存的脐血样本中的调节性 T 细胞进行计数。
PLoS One. 2020 Oct 23;15(10):e0240190. doi: 10.1371/journal.pone.0240190. eCollection 2020.
2
Cord blood attached segment: is this a relevant quality control to predict a good hematopoietic stem cell graft?脐带血附着片段:这是预测良好造血干细胞移植的相关质量控制指标吗?
Bone Marrow Transplant. 2017 Sep;52(9):1353-1354. doi: 10.1038/bmt.2017.150. Epub 2017 Jul 10.
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Molecular characterization of microbial contaminants isolated from Umbilical Cord Blood Units for transplant.
从用于移植的脐带血单位中分离出的微生物污染物的分子特征分析。
Braz J Infect Dis. 2015 Nov-Dec;19(6):571-7. doi: 10.1016/j.bjid.2015.07.005. Epub 2015 Sep 9.
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Detecting primitive hematopoietic stem cells in total nucleated and mononuclear cell fractions from umbilical cord blood segments and units.从脐带血段和单位的总核细胞和单核细胞部分中检测原始造血干细胞。
J Transl Med. 2015;13:94. doi: 10.1186/s12967-015-0434-z. Epub 2015 Mar 18.