多糖键合手性固定相的合成及其在高效液相色谱手性拆分中的应用。
Synthesis and application of immobilized polysaccharide-based chiral stationary phases for enantioseparation by high-performance liquid chromatography.
机构信息
Polymer Materials Research Center, Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Materials Science and Chemical Engineering, Harbin Engineering University, Harbin 150001, PR China.
Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
出版信息
J Chromatogr A. 2014 Oct 10;1363:51-61. doi: 10.1016/j.chroma.2014.06.042. Epub 2014 Jun 22.
Polysaccharide-based chiral stationary phases (CSPs) or chiral packing materials (CPMs) have been frequently employed for analyzing and separating various enantiomers by high-performance liquid chromatography (HPLC). The polysaccharide derivatives dissolved in a solvent are usually coated on silica gel to be used as CSPs. This means that some solvents, which can swell or dissolve the derivatives, cannot be used as the eluents in HPLC. In this review, various immobilization methods of the polysaccharide derivatives are described. The immobilization often reduces the chiral recognition ability compared to that of the corresponding coated-type CSPs. This problem can be overcome by the versatility of eluent selection for the immobilized CSPs. Enantioseparations of various racemates on the immobilized commercial columns using the non-standard eluents are briefly summarized.
多糖手性固定相（CSPs）或手性填充材料（CPMs）常用于通过高效液相色谱（HPLC）分析和分离各种对映异构体。多糖衍生物溶解在溶剂中，通常涂覆在硅胶上作为 CSPs 使用。这意味着一些能够溶胀或溶解衍生物的溶剂不能用作 HPLC 中的洗脱剂。在这篇综述中，描述了多糖衍生物的各种固定化方法。与相应的涂覆型 CSPs 相比，固定化通常会降低手性识别能力。通过固定化 CSPs 洗脱剂选择的多功能性可以克服这个问题。简要总结了使用非标准洗脱剂在固定化商业柱上对各种外消旋体的对映体分离。
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