超分子亲和分离的最新进展：亲和色谱及相关方法
Recent Advances in Supramolecular Affinity Separations: Affinity Chromatography and Related Methods.
作者信息
Woolfork Ashley G, Iftekhar Sazia, Ovbude Susan, Suh Kyungah, Sharmeen Sadia, Kyei Isaac, Jones Jacob, Hage David S
机构信息
Department of Chemistry, University of Nebraska, Lincoln, NE 68588 (USA).
出版信息
Adv Chromatogr. 2021;58:1-74. doi: 10.1201/9781003223405-1.
Affinity chromatography is a technique that uses a stationary phase based on the supramolecular interactions that occur in biological systems or mimics of these systems. This method has long been a popular tool for the isolation, measurement, and characterization of specific targets in complex samples. This review discusses the basic concepts of this method and examines recent developments in affinity chromatography and related supramolecular separation methods. Topics that are examined include advances that have occurred in the types of supports, approaches to immobilization, and binding agents that are employed in this method. New developments in the applications of affinity chromatography are also summarized, including an overview on the use of this method for biochemical purification, sample preparation or analysis, chiral separations, and biointeraction studies.
亲和色谱法是一种利用基于生物系统中发生的超分子相互作用或这些系统的模拟物的固定相的技术。长期以来，该方法一直是用于分离、测量和表征复杂样品中特定目标的常用工具。本文综述讨论了该方法的基本概念，并研究了亲和色谱法及相关超分子分离方法的最新进展。所探讨的主题包括该方法中使用的载体类型、固定化方法和结合剂方面取得的进展。还总结了亲和色谱法应用的新进展，包括对该方法用于生化纯化、样品制备或分析、手性分离和生物相互作用研究的概述。
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