Li Shuo, Jia Zirui, Li Xuejing, Geng Xuyun, Sun Jinsheng
Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, 393 Binshuixidao, Xiqing District, Tianjin 300387, China.
Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, 393 Binshuixidao, Xiqing District, Tianjin 300387, China.
Fish Shellfish Immunol. 2014 Sep;40(1):120-8. doi: 10.1016/j.fsi.2014.06.027. Epub 2014 Jul 2.
Calmodulin (CaM) is a multifunctional calcium sensor protein that participates in various cellular processes under normal, stress and pathological conditions. In crabs, however, the involvement of CaM in response to environmental stress and immune challenges has not been revealed yet. In the present study, a CaM cDNA (EsCaM) was identified from Chinese mitten crab Eriocheir sinensis and its mRNA expression patterns in response to ambient (salinity and pH) stress and immune challenges was examined. EsCaM encodes a 149-amino-acid protein with a calculated molecular mass of 16.8 kDa and an isoelectric point of 4.09. In unstimulated healthy E. sinensis, EsCaM mRNA transcript was detected in all tested tissues with predominant expression in hepatopancreas and the lowest expression in haemocytes. Ambient salinity (15‰ and 30‰ salinities) and pH (pH 6 and 8.5) stress significantly altered EsCaM mRNA expression in gill, hepatopancreas, haemocytes, intestine and muscle in Chinese mitten crab. In addition, EsCaM gene expression was significantly and rapidly induced as early as 2 h after LPS and Poly(I:C) immune stimulations in haemocytes in vitro. Furthermore, EsCaM expression was significantly up-regulated in E. sinensis haemocytes, gill, hepatopancreas, intestine and muscle in response to Edwardsiella tarda and Vibrio anguillarum challenges. Collectively, our findings suggest that EsCaM is an important stress and immune response gene in Chinese mitten crab.
钙调蛋白（CaM）是一种多功能钙传感蛋白，在正常、应激和病理条件下参与各种细胞过程。然而，在螃蟹中，CaM在应对环境应激和免疫挑战中的作用尚未明确。在本研究中，从中华绒螯蟹中鉴定出一个CaM cDNA（EsCaM），并检测了其在应对环境（盐度和pH值）应激和免疫挑战时的mRNA表达模式。EsCaM编码一种149个氨基酸的蛋白质，计算分子量为16.8 kDa，等电点为4.09。在未受刺激的健康中华绒螯蟹中，在所有测试组织中均检测到EsCaM mRNA转录本，其中肝胰腺中表达量最高，血细胞中表达量最低。环境盐度（15‰和30‰盐度）和pH值（pH 6和8.5）应激显著改变了中华绒螯蟹鳃、肝胰腺、血细胞、肠道和肌肉中EsCaM mRNA的表达。此外，在体外血细胞中，LPS和Poly（I:C）免疫刺激后2小时，EsCaM基因表达就被显著快速诱导。此外，在迟缓爱德华氏菌和鳗弧菌的刺激下，中华绒螯蟹血细胞、鳃、肝胰腺、肠道和肌肉中的EsCaM表达显著上调。总的来说，我们的研究结果表明，EsCaM是中华绒螯蟹中一个重要的应激和免疫反应基因。
