Patel Deepti Manjari, Brinchmann Monica F
Faculty of Biosciences and Aquaculture, Nord University, 8049 Bodø, Norway.
Biochem Biophys Rep. 2017 Jan 5;9:217-225. doi: 10.1016/j.bbrep.2016.12.016. eCollection 2017 Mar.
Fish skin mucus serves as a first line of defense against pathogens and external stressors. In this study the proteomic profile of lumpsucker skin mucus was characterized using 2D gels coupled with tandem mass spectrometry. Mucosal proteins were identified by homology searches across the databases SwissProt, NCBInr and vertebrate EST. The identified proteins were clustered into ten groups based on their gene ontology biological process in PANTHER (www.patherdb.org). Calmodulin, cystatin-B, histone H2B, peroxiredoxin1, apolipoprotein A1, natterin-2, 14-3-3 protein, alfa enolase, pentraxin, warm temperature acclimation 65 kDa (WAP65kDa) and heat shock proteins were identified. Several of the proteins are known to be involved in immune and/or stress responses. Proteomic profile established in this study could be a benchmark for differential proteomics studies.
鱼皮黏液是抵御病原体和外部应激源的第一道防线。在本研究中，利用二维凝胶结合串联质谱对圆鳍鱼皮黏液的蛋白质组图谱进行了表征。通过在SwissProt、NCBInr和脊椎动物EST数据库中进行同源性搜索来鉴定黏膜蛋白。根据所鉴定蛋白质在PANTHER（www.patherdb.org）中的基因本体生物学过程，将其分为十组。鉴定出了钙调蛋白、胱抑素-B、组蛋白H2B、过氧化物酶1、载脂蛋白A1、纳特林-2、14-3-3蛋白、α烯醇化酶、五聚素、暖温驯化65 kDa（WAP65kDa）和热休克蛋白。已知其中几种蛋白质参与免疫和/或应激反应。本研究建立的蛋白质组图谱可为差异蛋白质组学研究提供基准。
