Ye Qing, Ding Shao-Feng, Wang Zhi-An, Feng Jie, Tan Wen-Bin
Department of Ophthalmology, Jining First People's Hospital, Jining, China E-mail :
Asian Pac J Cancer Prev. 2014;15(12):4995-5000. doi: 10.7314/apjcp.2014.15.12.4995.
Cancer constitutes a key pressure on public health regardless of the economy state in different countries. As a kind of highly malignant epithelial tumor, lacrimal gland adenoid cystic carcinoma can occur in any part of the body, such as salivary gland, submandibular gland, trachea, lung, breast, skin and lacrimal gland. Chemotherapy is one of the key treatment techniques, but drug resistance, especially MDR, seriously blunts its effects. As an element of the 60S large ribosomal subunit, the ribosomal protein L39-L gene appears to be documented specifically in the human testis and many human cancer samples of different origins.
Total RNA of cultured drug-resistant and susceptible lacrimal gland adenoid cystic carcinoma cells was seperated, and real time quantitative RT-PCR were used to reveal transcription differences between amycin resistant and susceptible strains of lacrimal gland adenoid cystic carcinoma cells. Viability assays were used to present the amycin resistance difference in a RPL39-L transfected lacrimal gland adenoid cystic carcinoma cell line as compared to control vector and null-transfected lacrimal gland adenoid cystic carcinoma cell lines.
The ribosomal protein L39-L transcription level was 6.5-fold higher in the drug-resistant human lacrimal gland adenoid cystic carcinoma cell line than in the susceptible cell line by quantitative RT-PCR analysis. The ribosomal protein L39-L transfected cells revealed enhanced drug resistance compared to plasmid vector-transfected or null-transfected cells as determined by methyl tritiated thymidine (3H-TdR) incorporation.
The ribosomal protein L39-L gene could possibly have influence on the drug resistance mechanism of lacrimal gland adenoid cystic carcinoma cells.
无论不同国家的经济状况如何，癌症都是公共卫生面临的关键压力。泪腺腺样囊性癌作为一种高度恶性的上皮性肿瘤，可发生于身体的任何部位，如唾液腺、颌下腺、气管、肺、乳腺、皮肤和泪腺。化疗是关键治疗技术之一，但耐药性，尤其是多药耐药性，严重削弱了其疗效。核糖体蛋白L39-L基因作为60S大核糖体亚基的一个元件，似乎仅在人类睾丸和许多不同来源的人类癌症样本中有记录。
分离培养的耐药和敏感泪腺腺样囊性癌细胞的总RNA，采用实时定量逆转录聚合酶链反应（RT-PCR）揭示泪腺腺样囊性癌细胞耐阿霉素和敏感菌株之间的转录差异。采用活力测定法，呈现与对照载体和空转染的泪腺腺样囊性癌细胞系相比，RPL39-L转染的泪腺腺样囊性癌细胞系中阿霉素耐药性的差异。
通过定量RT-PCR分析，耐药的人泪腺腺样囊性癌细胞系中核糖体蛋白L39-L的转录水平比敏感细胞系高6.5倍。通过甲基化氚标记胸腺嘧啶核苷（3H-TdR）掺入法测定，与质粒载体转染或空转染的细胞相比，核糖体蛋白L39-L转染的细胞显示出增强的耐药性。
核糖体蛋白L39-L基因可能对泪腺腺样囊性癌细胞的耐药机制有影响。
