三碘甲状腺原氨酸对大鼠载脂蛋白A-I、C-III和A-IV基因与核基质结合的影响。

The effect of triiodothyronine on the association of the rat apolipoprotein A-I, C-III and A-IV genes with the nuclear matrix.

作者信息

Apostolopoulos J J, La Scala M J, Shen P Y, Howlett G J

机构信息

Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Australia.

出版信息

Biochem Biophys Res Commun. 1989 Jun 15;161(2):576-82. doi: 10.1016/0006-291x(89)92638-7.

DOI:10.1016/0006-291x(89)92638-7

PMID:2500121

Abstract

The association of apolipoprotein genes with the nuclear matrix of rat liver has been studied. The proportion of the apolipoprotein (apo) A-I, A-IV, C-III and E genes and the albumin gene associated with the matrix in normal rat liver was approximately 11%. In hypothyroid rats, the association of the apo A-I and apo A-IV genes with the matrix was reduced to 1.3% and 2.1% respectively, while in hyperthyroid rats, the association increased to 59% and 39% respectively. In contrast, the association of the apo C-III gene, which is located between the A-I and A-IV genes on chromosome 11, was not significantly affected by thyroid status. The association of the apo E and albumin genes with the matrix was also unaltered by changes in thyroid status. The increased association of the apo A-I and A-IV genes with the nuclear matrix of hyperthyroid animals accompanies the transcriptional activation of these genes by thyroid hormones.

摘要

对大鼠肝脏载脂蛋白基因与核基质之间的关联进行了研究。正常大鼠肝脏中，载脂蛋白（apo）A-I、A-IV、C-III和E基因以及白蛋白基因与核基质的关联比例约为11%。在甲状腺功能减退的大鼠中，apo A-I和apo A-IV基因与核基质的关联分别降至1.3%和2.1%，而在甲状腺功能亢进的大鼠中，该关联分别增至59%和39%。相比之下，位于11号染色体上A-I和A-IV基因之间的apo C-III基因与核基质的关联并未受到甲状腺状态的显著影响。apo E基因和白蛋白基因与核基质的关联也不会因甲状腺状态的变化而改变。甲状腺功能亢进动物中apo A-I和A-IV基因与核基质的关联增加，这与甲状腺激素对这些基因的转录激活相伴发生。