三碘甲状腺原氨酸对大鼠载脂蛋白A-I和A-IV基因转录的影响。

The effect of triiodothyronine on rat apolipoprotein A-I and A-IV gene transcription.

作者信息

Apostolopoulos J J, La Scala M J, Howlett G J

机构信息

Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Australia.

出版信息

Biochem Biophys Res Commun. 1988 Aug 15;154(3):997-1002. doi: 10.1016/0006-291x(88)90238-0.

DOI:10.1016/0006-291x(88)90238-0

PMID:3136772

Abstract

The effects of triiodothyronine administration on the mRNA levels of apolipoprotein (apo)A-I, apo A-IV and albumin were determined in extracts of rat liver by hybridization to specific cDNA. The mRNA levels for apo A-I and apo A-IV in the rat liver increased to 347% and 143% of normal, respectively, following a single injection of triiodothyronine (50 microgram/100g body weight). Under the same conditions there was no significant change in the hepatic mRNA level for albumin. The rates of transcription of these genes were measured in isolated hepatic nuclei prepared from hypothyroid and hyperthyroid rats. The rate of transcription of the albumin gene did not change significantly in either thyroid state. The rate of transcription of the apo A-I and apo A-IV genes increased in the hyperthyroid state to 342% and 202% of normal respectively, and decreased in the hypothyroid state to 26% and 44% of normal respectively.

摘要

通过与特异性cDNA杂交，在大鼠肝脏提取物中测定了给予三碘甲状腺原氨酸对载脂蛋白（apo）A-I、apo A-IV和白蛋白mRNA水平的影响。单次注射三碘甲状腺原氨酸（50微克/100克体重）后，大鼠肝脏中apo A-I和apo A-IV的mRNA水平分别升至正常水平的347%和143%。在相同条件下，肝脏中白蛋白的mRNA水平无显著变化。在从甲状腺功能减退和甲状腺功能亢进大鼠制备的分离肝细胞核中测量了这些基因的转录速率。在两种甲状腺状态下，白蛋白基因的转录速率均无显著变化。在甲状腺功能亢进状态下，apo A-I和apo A-IV基因的转录速率分别升至正常水平的342%和202%，而在甲状腺功能减退状态下，分别降至正常水平的26%和44%。