Coppens Joeri, Decostere Bjorge, Van Hulle Stijn, Nopens Ingmar, Vlaeminck Siegfried E, De Gelder Leen, Boon Nico
Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, 9000, Gent, Belgium.
Appl Microbiol Biotechnol. 2014 Oct;98(19):8377-87. doi: 10.1007/s00253-014-5854-9. Epub 2014 Jul 9.
Within sustainable resource management, the recovery of nitrogen and phosphorus nutrients from waste streams is becoming increasingly important. Although the use of microalgae has been described extensively in environmental biotechnology, the potential of nitrate-accumulating microalgae for nutrient recovery has not been investigated yet. The ability of these marine microorganisms to concentrate environmental nitrate within their biomass is remarkable. The aim of this study was to investigate the application potential of nitrate-accumulating diatoms for nutrient recovery from marine wastewaters. The intracellular nitrate storage capacity was quantified for six marine diatom strains in synthetic wastewater. Amphora coffeaeformis and Phaeodactylum tricornutum stored the highest amount of nitrate with respectively 3.15 and 2.10 g N L(-1) of cell volume, which accounted for 17.3 and 4.6 %, respectively, of the total nitrogen content. The growth and nitrate and phosphate uptake of both diatoms were further analyzed and based on these features P. tricornutum showed the highest potential for nutrient recovery. A mathematical model was developed which included intracellular nitrate storage and the kinetic parameters were derived for P. tricornutum. Furthermore, a simulation study was performed to compare the performance of a proposed microalgal nutrient recovery unit with a conventional denitrification system for marine wastewater treatment. Overall, this study demonstrates the potential application of P. tricornutum for saline wastewater treatment with concurrent nitrogen and phosphorus recycling.
在可持续资源管理中，从废水中回收氮和磷养分变得越来越重要。尽管微藻在环境生物技术中的应用已被广泛描述，但硝酸盐积累微藻在养分回收方面的潜力尚未得到研究。这些海洋微生物在其生物量中浓缩环境硝酸盐的能力非常显著。本研究的目的是研究硝酸盐积累硅藻在从海水中回收养分方面的应用潜力。对六种海洋硅藻菌株在合成废水中的细胞内硝酸盐储存能力进行了定量。咖啡双眉藻和三角褐指藻储存的硝酸盐量最高，分别为每升细胞体积3.15克氮和2.10克氮，分别占总氮含量的17.3%和4.6%。进一步分析了这两种硅藻的生长以及硝酸盐和磷酸盐的吸收情况，基于这些特征，三角褐指藻显示出最高的养分回收潜力。建立了一个包含细胞内硝酸盐储存的数学模型，并推导了三角褐指藻的动力学参数。此外，进行了一项模拟研究，以比较拟议的微藻养分回收装置与传统反硝化系统处理海水的性能。总体而言，本研究证明了三角褐指藻在同时进行氮和磷循环的含盐废水处理中的潜在应用。
