Guangdong Technology Research Center for Marine Algal Bioengineering, Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Longhua Innovation Institute for Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
College of Materials Science and Engineering, Chongqing University, Chongqing 400044, China.
Water Res. 2024 May 1;254:121430. doi: 10.1016/j.watres.2024.121430. Epub 2024 Mar 5.
Proper treatment of hypersaline and nutrient-rich food industry process water (FIPW) is challenging in conventional wastewater plants. Insufficient treatment leads to serious environmental hazards. However, bioremediation of FIPW with an indigenous microbial community can not only recover nutrients but generate biomass of diverse applications. In this study, monoculture of Halamphora coffeaeformis, together with synthetic bacteria isolated from a local wastewater plant, successfully recovered 91% of NH-N, 78% of total nitrogen, 95% of total phosphorus as well as 82% of total organic carbon from medium enriched with 10% FIPW. All identified organic acids and amino acids, except oxalic acid, were completely removed after 14 days treatment. A significantly higher biomass concentration (1.74 g L) was achieved after 14 days treatment in the medium with 10% FIPW than that in a nutrient-replete lab medium as control. The harvested biomass could be a potential feedstock for high-value biochemicals and fertilizer production, due to fucoxanthin accumulation (3 mg g) and a fantastic performance in P assimilation. Metagenomic analysis revealed that bacteria community in the algal system, dominated by Psychrobacter and Halomonas, also contributed to the biomass accumulation and uptake of nutrients. Transcriptomic analysis further disclosed that multiple pathways, involved in translation, folding, sorting and degradation as well as transport and catabolism, were depressed in H. coffeaeformis grown in FIPW-enriched medium, as compared to the control. Collectively, the proposed one-step strategy in this work offers an opportunity to achieve sustainable wastewater management and a way towards circular economy.
在传统废水处理厂中，对高盐和富含营养的食品工业工艺废水（FIPW）进行妥善处理具有挑战性。处理不充分会导致严重的环境危害。然而，利用本地微生物群落对 FIPW 进行生物修复不仅可以回收营养物质，还可以生成具有多种应用的生物质。在这项研究中，Halamphora coffeaeformis 的纯培养物，以及从当地废水厂分离的合成细菌，成功地从富含 10%FIPW 的培养基中回收了 91%的 NH-N、78%的总氮、95%的总磷和 82%的总有机碳。经过 14 天的处理，所有鉴定出的有机酸和氨基酸（除了草酸）都被完全去除。在含有 10%FIPW 的培养基中处理 14 天后，生物量浓度（1.74 g/L）显著高于作为对照的营养丰富的实验室培养基。由于褐藻黄素的积累（3 mg/g）和在磷吸收方面的出色表现，收获的生物质可以成为高价值生物化学物质和肥料生产的潜在原料。宏基因组分析表明，藻类系统中的细菌群落，主要由 Psychrobacter 和 Halomonas 组成，也有助于生物质的积累和营养物质的吸收。转录组分析进一步揭示了与翻译、折叠、分类和降解以及运输和分解代谢相关的多个途径在 H. coffeaeformis 生长在富含 FIPW 的培养基中时被抑制，与对照相比。总之，这项工作中提出的一步策略为实现可持续的废水管理和循环经济提供了机会。
