Wang Xiaofei, Liu Junlian, Song Shujun, Xu Bingxin, Tan Xiaoqing, Si Shaoyan
No.306 Clinical College of PLA, Anhui Medical University, Beijing 100101, China.
Department of Dermatology, No.306 Hospital of PLA, Beijing 100101, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2014 Jul;30(7):736-9.
To detect the expressions of Toll-like receptor 2 (TLR2), TLR7, TLR9 and dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN) mRNA and protein in peripheral blood mononuclear cells (PBMCs) from patients with chronic urticaria (CU).
PBMCs were isolated from 20 patients with CU and 20 normal controls. The expressions of TLR2, TLR7, TLR9 and DC-SIGN mRNA were detected by real-time fluorescent quantitative PCR and the expressions of their proteins were detected by flow cytometry.
The expression of DC-SIGN mRNA in PBMCs from patients with CU was significantly lower than that from normal controls (P<0.01). The expression of TLR2 protein was significantly higher (P<0.05), but the expression of DC-SIGN protein was significantly lower (P<0.05) in CU patients than those in normal controls.
The expression of DC-SIGN decreases and that of TLR2 protein increases in patients with CU.
检测慢性荨麻疹（CU）患者外周血单个核细胞（PBMCs）中Toll样受体2（TLR2）、TLR7、TLR9和树突状细胞特异性细胞间黏附分子3结合非整合素（DC-SIGN）mRNA及蛋白的表达。
从20例CU患者和20例正常对照者中分离PBMCs。采用实时荧光定量PCR检测TLR2、TLR7、TLR9和DC-SIGN mRNA的表达，采用流式细胞术检测其蛋白表达。
CU患者PBMCs中DC-SIGN mRNA的表达明显低于正常对照者（P<0.01）。CU患者中TLR2蛋白的表达明显升高（P<0.05），但DC-SIGN蛋白的表达明显低于正常对照者（P<0.05）。
CU患者DC-SIGN表达降低，TLR2蛋白表达升高。
