Zheng Lingyan, Zhang Zhiyuan, Yu Chuangqi, Yang Chi
Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, School of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Jun;109(6):844-50. doi: 10.1016/j.tripleo.2010.01.006.
The objective of this study was to investigate the level of Toll-like receptors (TLRs) 7, 8, and 9 in peripheral mononuclear blood cells (PMBCs) from patients with primary Sjögren's syndrome (pSS) and whether TLR7 and 9 exist in the parotid glands of pSS patients.
TLR7, 8, and 9 mRNA levels in PMBC from 37 pSS patients and 24 controls were determined using real-time polymerase chain reaction. TLR7- and TLR9-positive cells in parotid glands from 20 pSS patients and 10 controls were observed using immunofluorescence and immunohistochemistry, respectively. Statistical analysis was performed by Student t test.
TLR7 and TLR9 mRNA levels were 2.17- and 2.33-fold higher in pSS patients than control subjects, respectively (P < .05), whereas TLR8 levels were not. TLR7- and TLR9-positive cells of the pSS group were localized in the epithelial islands, lymphocytes, and ductal epithelial cells of the parotid glands and were more abundant than the TLR7- and TLR9-positive cells that were only localized to the ductal epithelial cells of parotid glands (P < .05).
TLR7 and TLR9 mRNA levels are up-regulated in the PMBC of pSS patients, and TLR7- and TLR9-positive cells exist in the epithelial islands, lymphocytes, and ductal epithelial cells of the parotid glands of individuals affected by pSS, but are limited to the ductal epithelial cells of controls.
本研究旨在调查原发性干燥综合征（pSS）患者外周血单个核细胞（PMBCs）中Toll样受体（TLRs）7、8和9的水平，以及pSS患者腮腺中是否存在TLR7和9。
采用实时聚合酶链反应测定37例pSS患者和24例对照者PMBC中TLR7、8和9的mRNA水平。分别采用免疫荧光和免疫组化方法观察20例pSS患者和10例对照者腮腺中TLR7和TLR9阳性细胞。采用Student t检验进行统计学分析。
pSS患者TLR7和TLR9的mRNA水平分别比对照者高2.17倍和2.33倍（P <.05），而TLR8水平无差异。pSS组TLR7和TLR9阳性细胞定位于腮腺的上皮岛、淋巴细胞和导管上皮细胞，比仅定位于腮腺导管上皮细胞的TLR7和TLR9阳性细胞更丰富（P <.05）。
pSS患者PMBC中TLR7和TLR9的mRNA水平上调，pSS患者腮腺的上皮岛、淋巴细胞和导管上皮细胞中存在TLR7和TLR9阳性细胞，但对照者仅局限于导管上皮细胞。
