Williams Maya, Mayer Sandra V, Johnson William L, Chen Rubing, Volkova Evgeniya, Vilcarromero Stalin, Widen Steven G, Wood Thomas G, Suarez-Ognio Luis, Long Kanya C, Hanley Kathryn A, Morrison Amy C, Vasilakis Nikos, Halsey Eric S
Department of Virology, US Naval Medical Research Unit No. 6, Lima, Peru; Department of Pathology and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas; Department of Biology, New Mexico State University, Las Cruces, New Mexico; Department of Virology, US Naval Medical Research Unit No. 6, Iquitos, Peru; Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas; Dirección General de Epidemiología del Ministerio de Salud del Perú, Lima, Perú; Entomology Department, University of California, Davis, California; Institute for Human Infections and Immunity and Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas
Department of Virology, US Naval Medical Research Unit No. 6, Lima, Peru; Department of Pathology and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas; Department of Biology, New Mexico State University, Las Cruces, New Mexico; Department of Virology, US Naval Medical Research Unit No. 6, Iquitos, Peru; Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas; Dirección General de Epidemiología del Ministerio de Salud del Perú, Lima, Perú; Entomology Department, University of California, Davis, California; Institute for Human Infections and Immunity and Center for Tropical Diseases, University of Texas Medical Branch, Galveston, Texas.
Am J Trop Med Hyg. 2014 Sep;91(3):611-20. doi: 10.4269/ajtmh.13-0600. Epub 2014 Jul 7.
During 2010 and 2011, the Loreto region of Peru experienced a dengue outbreak of unprecedented magnitude and severity for the region. This outbreak coincided with the reappearance of dengue virus-2 (DENV-2) in Loreto after almost 8 years. Whole-genome sequence indicated that DENV-2 from the outbreak belonged to lineage II of the southeast Asian/American genotype and was most closely related to viruses circulating in Brazil during 2007 and 2008, whereas DENV-2 previously circulating in Loreto grouped with lineage I (DENV-2 strains circulating in South America since 1990). One amino acid substitution (NS5 A811V) in the 2010 and 2011 isolates resulted from positive selection. However, the 2010 and 2011 DENV-2 did not replicate to higher titers in monocyte-derived dendritic cells and did not infect or disseminate in a higher proportion of Aedes aegypti than DENV-2 isolates previously circulating in Loreto. These results suggest that factors other than enhanced viral replication played a role in the severity of this outbreak.
在2010年和2011年期间，秘鲁的洛雷托地区经历了一次该地区前所未有的大规模且严重的登革热疫情。此次疫情恰逢登革热病毒2型（DENV-2）在洛雷托地区几乎8年后再次出现。全基因组序列表明，此次疫情中的DENV-2属于东南亚/美洲基因型的第二分支，与2007年和2008年在巴西传播的病毒关系最为密切，而此前在洛雷托地区传播的DENV-2则与第一分支（自1990年以来在南美洲传播的DENV-2毒株）归为一类。2010年和2011年分离出的毒株中有一个氨基酸替换（NS5 A811V）是阳性选择的结果。然而，2010年和2011年的DENV-2在单核细胞衍生的树突状细胞中复制到更高滴度的能力并不强，在埃及伊蚊中的感染或传播比例也不比此前在洛雷托地区传播的DENV-2分离株高。这些结果表明，病毒复制增强以外的因素在此次疫情的严重程度中起到了作用。
