Hedbys L, Johansson E, Mosbach K, Larsson P O
Department of Pure and Applied Biochemistry, University of Lund, Sweden.
Carbohydr Res. 1989 Mar 15;186(2):217-23. doi: 10.1016/0008-6215(89)84036-4.
beta-D-Galp-(1----3)-D-GalNAc (1) was synthesised from lactose and GalNAc on a mmolar scale by transgalactosylation using beta-D-galactosidase from bovine testes. The large proportions of unwanted oligosaccharides in the product mixture were removed by treatment with beta-D-galactosidase from E. coli, which left 1, monosaccharides, and a small proportion of trisaccharides. Carbon-Celite chromatography then gave 1 in a yield of 21% based on the GalNAc added.
β-D-半乳糖基-（1→3）-D-氨基半乳糖（1）由乳糖和氨基半乳糖以毫摩尔规模通过使用来自牛睾丸的β-D-半乳糖苷酶进行转半乳糖基化反应合成。产物混合物中大量不需要的低聚糖通过用来自大肠杆菌的β-D-半乳糖苷酶处理而被去除，剩余物为1、单糖和一小部分三糖。然后通过碳-硅藻土色谱法，基于添加的氨基半乳糖，以21%的产率得到1。
