Department of Clinical and Experimental Medicine, Unit of Autoimmunity and Immune Regulation, Division of Clinical Immunology, Linköping University, SE-581 85 Linköping, Sweden.
Department of Clinical and Experimental Medicine, Division of Pediatrics, Linköping University, Linköping, Sweden.
Clin Transl Allergy. 2014 Jun 25;4:21. doi: 10.1186/2045-7022-4-21. eCollection 2014.
Mice models indicate that intact Toll like receptor (TLR) signaling may be essential for the allergy protective effects of diverse bacterial exposure observed in clinical trials and epidemiological studies. Probiotic supplementation with Lactobacillus reuteri from pregnancy week 36 and to the infant through the first year of life decreased the prevalence of IgE-associated eczema at two years (ClinicalTrials.gov NCT01285830). The effect of this supplementation on innate immune responses to bacterial products and the expression of associated TLRs were explored.
Blood mononuclear cells were collected at birth, 6, 12 and 24 months from 61 infants and cultured with TLR2, 4 and 9 ligands. Cytokine and chemokine secretion was determined as well as TLR2, 4 and 9 mRNA expression.
Probiotic supplementation was associated with decreased LTA (lipoteichoic acid) induced CCL4, CXCL8, IL-1β and IL-6 responses at 12 months and decreased CCL4 and IL-1β secretion at 24 months. TLR2 mRNA expression was not affected by probiotic treatment.
Decreased responses to TLR2, the main receptor for LTA from Gram positive bacteria, in probiotic treated children seem to be dependent on factors downstream of TLR mRNA expression.
小鼠模型表明，完整的 Toll 样受体（TLR）信号可能对于临床试验和流行病学研究中观察到的各种细菌暴露的过敏保护作用至关重要。从妊娠第 36 周开始，用罗伊氏乳杆菌（Lactobacillus reuteri）对孕妇进行补充，并在婴儿出生后的第一年进行补充，可降低两岁时 IgE 相关湿疹的患病率（ClinicalTrials.gov NCT01285830）。本研究探索了这种补充对细菌产物的固有免疫反应和相关 TLR 表达的影响。
从 61 名婴儿的出生、6、12 和 24 个月时收集血单核细胞，并与 TLR2、4 和 9 配体一起培养。测定细胞因子和趋化因子的分泌以及 TLR2、4 和 9 mRNA 的表达。
补充益生菌与 12 个月时 LTA（脂磷壁酸）诱导的 CCL4、CXCL8、IL-1β和 IL-6 反应降低以及 24 个月时 CCL4 和 IL-1β 分泌降低有关。TLR2 基因表达不受益生菌治疗的影响。
在接受益生菌治疗的儿童中，TLR2（革兰氏阳性菌 LTA 的主要受体）反应降低似乎依赖于 TLR mRNA 表达下游的因素。
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