Birlouez-Aragon I, Alloussi S, Morawiec M, Fevrier C
Laboratoire de Nutrition Humaine, Institut National Agronomique, Paris, France.
Curr Eye Res. 1989 May;8(5):449-57. doi: 10.3109/02713688909000025.
The cataractogenic effect of a galactose diet was studied in male and female pigs in relation to the daily galactose intake. Two experiments were performed. In the first, 10 male and 10 female pigs were maintained on a 5% galactose diet for 30 days; galactose was given either as pure sugar or as hydrolyzed whey. In the second experiment, 18 castrated male and 21 female pigs were fed a 25% galactose diet for 49 days. Galactose was given as whey, hydrolyzed whey or as an alternating diet. Lenses were analyzed for sugar-alcohols, glutathione and protein glycation, and compared to lenses of pigs on a control standard diet. The 5% galactose diet induced large accumulation of dulcitol in the lens, which was similar whether galactose was ingested alone or as hydrolyzed whey. Glutathione and inositol contents were slightly below control values only in males on the galactose alone diet, perhaps indicating initiation of a cataractogenic process. No change was observed in females. The lenses of females on control diet were different from those of control males: having decreased glutathione, inositol and sorbitol contents and higher protein glycation. The 25% galactose diet resulted in an approximately 10 times higher lens dulcitol accumulation and advanced lens damage as measured by loss of inositol and increase in protein glycation. These changes were more severe in males than in females. The data indicate that there is a relationship between total galactose intake and dulcitol accumulation.
研究了半乳糖饮食对雄性和雌性猪的致白内障作用与每日半乳糖摄入量的关系。进行了两项实验。在第一项实验中，10只雄性猪和10只雌性猪维持5%半乳糖饮食30天；半乳糖以纯糖或水解乳清的形式给予。在第二项实验中，18只去势雄性猪和21只雌性猪喂食25%半乳糖饮食49天。半乳糖以乳清、水解乳清或交替饮食的形式给予。分析晶状体中的糖醇、谷胱甘肽和蛋白质糖基化，并与喂食对照标准饮食的猪的晶状体进行比较。5%半乳糖饮食导致晶状体中大量积累卫矛醇，无论半乳糖是单独摄入还是以水解乳清的形式摄入，情况都相似。仅在单独喂食半乳糖的雄性猪中，谷胱甘肽和肌醇含量略低于对照值，这可能表明致白内障过程开始。在雌性猪中未观察到变化。对照饮食的雌性猪的晶状体与对照雄性猪的不同：谷胱甘肽、肌醇和山梨醇含量降低以及蛋白质糖基化程度更高。25%半乳糖饮食导致晶状体卫矛醇积累增加约10倍，并且通过肌醇损失和蛋白质糖基化增加来衡量，晶状体损伤加重。这些变化在雄性猪中比在雌性猪中更严重。数据表明总半乳糖摄入量与卫矛醇积累之间存在关系。
