Dethy J M, Callaert-Deveen B, Janssens M, Lenaers A
Anal Biochem. 1984 Nov 15;143(1):119-24. doi: 10.1016/0003-2697(84)90565-7.
Aldose reductase is an enzyme that plays an important role in diabetic complications such as cataract and neuropathy. The best way to estimate the enzymatic activity of this enzyme in vivo consists of measuring the accumulation of sorbitol or galactitol in various types of cells or tissues. A sensitive method to measure the polyols in biological samples by high-performance liquid chromatography has been developed. This method is based on the fact that polyols like sorbitol and galactitol react with phenylisocyanate to yield uv-absorbing derivatives at 240 nm. Applications to the separation and determination of polyols in biological samples of various origins, such as lenses, sciatic nerves, human skin fibroblasts, and red cells, are described and illustrated.
醛糖还原酶是一种在糖尿病并发症（如白内障和神经病变）中起重要作用的酶。在体内评估这种酶的酶活性的最佳方法是测量各种类型的细胞或组织中山梨醇或半乳糖醇的积累。已经开发出一种通过高效液相色谱法测量生物样品中多元醇的灵敏方法。该方法基于山梨醇和半乳糖醇等多元醇与异氰酸苯酯反应在240nm处产生可吸收紫外线的衍生物这一事实。描述并举例说明了该方法在分离和测定各种来源的生物样品（如晶状体、坐骨神经、人皮肤成纤维细胞和红细胞）中多元醇的应用。
