Xia Hui, Sun Shengjie, Wang Bo, Wang Tao, Liang Chaoyang, Li Guo, Huang Chongbiao, Qi Daliang, Chu Xiangyang
Department of Thoracic-Cardio Surgery, the First Affiliated Hospital of PLA General Hospital, Beijing 100048, China.
Department of Medical Oncology, the General Hospital of People's Liberation Army, Beijing 100853, China.
Int J Mol Sci. 2014 Jul 7;15(7):11973-83. doi: 10.3390/ijms150711973.
MicroRNAs (miRNAs) have essential roles in carcinogenesis and tumor progression. Here, we investigated the roles and mechanisms of miR-143 in non-small cell lung cancer (NSCLC). miR-143 was significantly decreased in NSCLC tissues and cell lines. Overexpression of miR-143 suppressed NSCLC cell proliferation, induced apoptosis, and inhibited migration and invasion in vitro. Integrated analysis identified LIM domain kinase 1 (Limk1) as a direct and functional target of miR-143. Overexpression of Limk1 attenuated the tumor suppressive effects of miR-143 in NSCLC cells. Moreover, miR-143 was inversely correlated with Limk1 expression in NSCLC tissues. Together, our results highlight the significance of miR-143 and Limk1 in the development and progression of NSCLC.
微小RNA（miRNA）在癌症发生和肿瘤进展中发挥着重要作用。在此，我们研究了miR-143在非小细胞肺癌（NSCLC）中的作用及机制。miR-143在NSCLC组织和细胞系中显著降低。miR-143的过表达抑制了NSCLC细胞增殖，诱导了细胞凋亡，并在体外抑制了细胞迁移和侵袭。综合分析确定LIM结构域激酶1（Limk1）是miR-143的直接功能靶点。Limk1的过表达减弱了miR-143对NSCLC细胞的肿瘤抑制作用。此外，miR-143在NSCLC组织中的表达与Limk1呈负相关。总之，我们的结果突出了miR-143和Limk1在NSCLC发生发展中的重要性。
