Duo Tianmeng, Goddard-Borger Ethan D, Withers Stephen G
The Department of Chemistry, The University of British Columbia, 2036 Main Mall, Vancouver, BC, Canada V6T 1Z1.
Chem Commun (Camb). 2014 Aug 25;50(66):9379-82. doi: 10.1039/c4cc03299c.
Novel fluorogenic 2-deoxy-2-fluoroglycosyl acridinone active site titrating reagents were synthesised and kinetic parameters determined for their inactivation of two retaining β-glucosidases, a β-galactosidase, a β-xylosidase and several cellulases. Fluorescence-monitored active site titration using this class of reagents reliably measured active enzyme concentrations down to 3 nM.
合成了新型荧光2-脱氧-2-氟糖基吖啶酮活性位点滴定试剂，并测定了它们对两种保留型β-葡萄糖苷酶、一种β-半乳糖苷酶、一种β-木糖苷酶和几种纤维素酶的失活动力学参数。使用这类试剂进行荧光监测的活性位点滴定能够可靠地测量低至3 nM的活性酶浓度。
