Zaazaa Hala E, Mohamed Afaf O, Hawwam Maha A, Abdelkawy Mohamed
Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt.
National Organization for Drug Control and Research (NODCAR), 6-Abu Hazem Street, Pyramids Ave., P.O. Box 29, 35521 Giza, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Jan 5;134:109-13. doi: 10.1016/j.saa.2014.06.058. Epub 2014 Jun 20.
A simple, sensitive and selective spectrofluorimetric method has been developed for the determination of 3-methylflavone-8-carboxylic acid as the main active metabolite of flavoxate hydrochloride in human urine. The proposed method was based on the measurement of the native fluorescence of the metabolite in methanol at an emission wavelength 390 nm, upon excitation at 338 nm. Moreover, the urinary excretion pattern has been calculated using the proposed method. Taking the advantage that 3-methylflavone-8-carboxylic acid is also the alkaline degradate, the proposed method was applied to in vitro determination of flavoxate hydrochloride in tablets dosage form via the measurement of its corresponding degradate. The method was validated in accordance with the ICH requirements and statistically compared to the official method with no significant difference in performance.
已开发出一种简单、灵敏且具选择性的荧光分光光度法，用于测定盐酸黄酮哌酯在人尿液中的主要活性代谢物3-甲基黄酮-8-羧酸。该方法基于在338nm激发下，于发射波长390nm处测量甲醇中代谢物的天然荧光。此外，已使用该方法计算了尿排泄模式。利用3-甲基黄酮-8-羧酸也是碱性降解产物这一优势，通过测量其相应的降解产物，将该方法应用于体外测定片剂剂型中的盐酸黄酮哌酯。该方法按照国际协调会议（ICH）要求进行了验证，并与官方方法进行了统计学比较，性能无显著差异。
