小鼠实验性自身免疫性脑脊髓炎
Experimental Autoimmune Encephalomyelitis in Mice.
作者信息
Terry Rachael L, Ifergan Igal, Miller Stephen D
机构信息
Department of Microbiology-Immunology and Interdepartmental Immunobiology Center, Feinberg School of Medicine, Northwestern University, Chicago, IL, 60611, USA.
出版信息
Methods Mol Biol. 2016;1304:145-60. doi: 10.1007/7651_2014_88.
Abstract
Experimental autoimmune encephalitis (EAE), the animal model of multiple sclerosis (MS), has provided significant insight into the mechanisms that initiate and drive autoimmunity. Several central nervous system proteins and peptides have been used to induce disease, in a number of different mouse strains, to model the diverse clinical presentations of MS. In this chapter, we detail the materials and methods used to induce active and adoptive EAE. We focus on disease induction in the SJL/J, C57BL/6, and BALB/c mouse strains, using peptides derived from proteolipid protein, myelin basic protein, and myelin oligodendrocyte glycoprotein. We also include a protocol for the isolation of leukocytes from the spinal cord and brain for flow cytometric analysis.
摘要
实验性自身免疫性脑脊髓炎（EAE）是多发性硬化症（MS）的动物模型，它为引发和驱动自身免疫的机制提供了重要见解。在许多不同的小鼠品系中，几种中枢神经系统蛋白和肽已被用于诱导疾病，以模拟MS的各种临床表现。在本章中，我们详细介绍了用于诱导主动型和过继型EAE的材料和方法。我们重点介绍了使用来自蛋白脂蛋白、髓鞘碱性蛋白和髓鞘少突胶质细胞糖蛋白的肽，在SJL/J、C57BL/6和BALB/c小鼠品系中诱导疾病。我们还包括了一个从脊髓和大脑中分离白细胞用于流式细胞术分析的方案。
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