Key Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, People's Republic of China; School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, People's Republic of China.
Key Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, People's Republic of China; School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People's Republic of China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, People's Republic of China.
Bioresour Technol. 2014 Sep;167:433-40. doi: 10.1016/j.biortech.2014.06.044. Epub 2014 Jun 19.
Cellular redox status and oxygen availability influence the product formation. Herein, decreasing agitation speed or adding vitamin C (Vc) achieved the 2,3-BDL yield of 0.40 g g(-1) or 0.39 g g(-1)glucose under batch fermentation, respectively. To our knowledge, this is the highest 2,3-BDL yield reported so far for Paenibacillus polymyxa without adding acetic acid. The NADH/NAD(+) ratio and 2,3-BDL titer could be increased significantly by reducing the agitation speed or adding Vc, indicating that the enhancement of 2,3-BDL is closely associated with the adjustment of NADH/NAD(+) ratio. Especially, Vc addition elevated the 2,3-BDL titer from 43.66 g L(-1) to 71.71 g L(-1) within 54 h under fed-batch fermentation. This is the highest titer of 2,3-BDL so far reported for P. polymyxa from glucose fermentation. This work provides a new strategy to improve 2,3-BDL production and helps us to understand the responses of P. polymyxa to extracellular oxidoreduction potential.
细胞氧化还原状态和氧气供应影响产物形成。在此，通过降低搅拌速度或添加维生素 C (Vc)，分别在分批发酵中实现了 2,3-BDL 的产率为 0.40 g g(-1)或 0.39 g g(-1)葡萄糖。据我们所知，这是迄今报道的高产 2,3-BDL 的最高产率，无需添加乙酸。通过降低搅拌速度或添加 Vc，可以显著提高 NADH/NAD(+) 比和 2,3-BDL 浓度，表明 2,3-BDL 的增强与 NADH/NAD(+) 比的调整密切相关。特别是，添加 Vc 可使 2,3-BDL 的浓度在 54 小时内从 43.66 g L(-1)提高到 71.71 g L(-1)，在补料分批发酵中。这是迄今为止报道的葡萄糖发酵中高产 2,3-BDL 的最高浓度，来自 P. polymyxa。这项工作为提高 2,3-BDL 的生产提供了新策略，并帮助我们了解 P. polymyxa 对细胞外氧化还原电势的反应。
