Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, 49 Soi Thianthale 25, Bangkhunthian-Chaithale Rd., Thakham, Bangkok 10150, Bangkhunthian, Thailand.
Bioresource Research Unit, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Phahonyothin Rd., Khlong Nueng, Klong Luang 12120, Pathum Thani, Thailand.
AMB Express. 2014 Jun 27;4:52. doi: 10.1186/s13568-014-0052-9. eCollection 2014.
Anhydromevalonolactone (AMVL) is a bioactive natural product that arises from a molecular biology technique using Aspergillus oryzae as a heterologous host. AMVL has been used as a precursor for the synthesis of insect pest control reagents and has numerous applications in the biotechnological and medical industries. In this study, the Plackett-Burman Design and the Central Composite Design, which offer efficient and feasible approaches, were complemented to screen significant parameters and identify the optimal values for maximum AMVL production. The results suggested that sucrose, NaNO3, yeast extract and K2HPO4 were the key factors affecting AMVL production in a complex medium, whereas the major components required for a defined medium were NaNO3, K2HPO4, KH2PO4 and trace elements. These factors were subsequently optimized using the response surface methodology. Under optimal conditions, a maximum AMVL production of 250 mg/L in the complex medium and 200 mg/L in the defined medium was achieved, which represents an increase of approximately 3-4-fold compared to the commonly used malt extract medium.
脱水马缨丹内酯（AMVL）是一种生物活性天然产物，它是利用米曲霉作为异源宿主的分子生物学技术产生的。AMVL 已被用作合成杀虫剂的前体，在生物技术和医药行业有许多应用。在这项研究中，Plackett-Burman 设计和中心复合设计作为有效且可行的方法得到了补充，用于筛选显著参数并确定最大 AMVL 产量的最佳值。结果表明，蔗糖、NaNO3、酵母提取物和 K2HPO4 是影响复杂培养基中 AMVL 产量的关键因素，而定义培养基中所需的主要成分是 NaNO3、K2HPO4、KH2PO4 和微量元素。这些因素随后通过响应面法进行了优化。在最佳条件下，在复杂培养基中的 AMVL 最大产量为 250mg/L，在定义培养基中的最大产量为 200mg/L，与常用的麦芽提取物培养基相比，产量增加了约 3-4 倍。
