Dzieciatkowski Tomasz, Przybylski Maciej, Rusicka Patrycja, Madry Krzysztof, Boguradzki Piotr, Marchel Halina, Jedrzejczak Wiesław W, Młynarczyk Grazyna
Med Dosw Mikrobiol. 2014;66(1):23-8.
Infections caused with a variety of bacteria, fungi and viruses are still responsible for high level of mortality and morbidity in immunosupressed individuals. A case of fatal post-transplant reactivation with four herpesviruses in 49-year-old immunocompromised male with MDS-RAEB2, subjected to allogeneic haematopoietic stem cell transplantation was described.
Full microbiological examination of was performed in different types of clinical samples (whole blood, stool). Sera specimens were tested for the presence of different viral DNA using the real-time PCR assays.
DNA of HSV-1, VZV, HHV-6 and EBV in serum samples was detected using molecular biology techniques. Viral level of HSV-1 and VZV was constantly increasing despite routine applied oral acyclovir therapy. These findings underline the value of real-time PCR technique used in current therapeutic procedures and for monitoring of antiviral therapy with nucleoside analogs. We found that real-time PCR is a useful tool in detection and monitoring of disseminated herpesviral infection, especially for the detection of low-copy viraemia in clinical specimens.
由多种细菌、真菌和病毒引起的感染仍然是免疫抑制个体高死亡率和高发病率的原因。本文描述了一例49岁患有MDS-RAEB2的免疫受损男性，在接受异基因造血干细胞移植后，四种疱疹病毒发生致命性再激活的病例。
对不同类型的临床样本（全血、粪便）进行全面的微生物学检查。使用实时PCR检测血清样本中不同病毒DNA的存在情况。
采用分子生物学技术在血清样本中检测到了HSV-1、VZV、HHV-6和EBV的DNA。尽管常规应用口服阿昔洛韦治疗，但HSV-1和VZV的病毒水平仍持续上升。这些发现强调了实时PCR技术在当前治疗程序以及监测核苷类似物抗病毒治疗中的价值。我们发现实时PCR是检测和监测播散性疱疹病毒感染的有用工具，特别是用于检测临床样本中的低拷贝病毒血症。
