Zhou Yue, Yang Bing, Yang Yang, Jia Rong
Sheng Wu Gong Cheng Xue Bao. 2014 Mar;30(3):524-8.
Manganese peroxidase (MnP), a crucial enzyme in lignin degradation, has wide potential applications in environmental protection. However, large-scale industrial application of this enzyme is limited due to several factors primarily related to cost and availability. Special attention has been paid to the production of MnP from inexpensive sources, such as lignocellulosic residues, using solid-state fermentation (SSF) systems. In the present study, a suitable SSF medium for the production of MnP by Schizophyllum sp. F17 from agro-industrial residues has been optimized. The mixed solid medium, comprising pine sawdust, rice straw, and soybean powder at a ratio of 0.52:0.15:0.33, conferred a maximum enzyme activity of 11.18 U/g on the sixth day of SSF. The results show that the use of wastes such as pine sawdust and rice straw makes the enzyme production more economical as well as helps solve environmental problems.
锰过氧化物酶（MnP）是木质素降解中的一种关键酶，在环境保护方面具有广泛的潜在应用。然而，由于一些主要与成本和可获得性相关的因素，这种酶的大规模工业应用受到限制。人们特别关注利用固态发酵（SSF）系统从廉价来源（如木质纤维素残渣）生产MnP。在本研究中，对裂褶菌F17利用农业工业残渣生产MnP的合适固态发酵培养基进行了优化。由松木锯末、稻草和大豆粉按0.52:0.15:0.33的比例组成的混合固体培养基，在固态发酵的第六天产生了最高酶活性，为11.18 U/g。结果表明，使用松木锯末和稻草等废料使酶的生产更经济，同时有助于解决环境问题。
