Pan Yan-Ting, Ao Ning-Jian, Shan Guang-Hua, Zhang Gang-Ping, Zhang Quan-Bin, Yang Ji-Wang, He Chun-Lan, Huang Yao-Xiong
Guang Pu Xue Yu Guang Pu Fen Xi. 2014 Apr;34(4):986-9.
Nano-silver colloid was synthesized by using microwave method on the mixtures of sodium citrate solution and silver nitrate solution. The method has advantages of fast heating speed, uniform temperature distribution and easily controlled reaction conditions. The sizes and size distributions of the silver particles were characterized by means of quasi-elastic laser scattering (QLS). The average particles size was (53.27 +/- 2.65) nm and the size of the particles was mainly distributed around 56 nm. Surface-enhanced Raman spectra of the degradation products from goat horn were obtained with silver colloid as active substrate. It was observed that the Raman signal of SERS was enhanced significantly compared with that of regular Raman spectrum, especially at the Raman bands of 659, 830, 850, 929, 999, 1 028, 1 280, 1 439 and 1 599 cm(-1) which reflect the biochemical components in degradation products. The characteristic Raman bands of degradation products from goat horn were preliminary assigned. The assignments showed that the main constituents of the degradation products from goat horn were amino acids and polypeptides. It was for the first time that Surface-enhanced Raman spectroscopy was used to detect trace degradation products from the horns. Raman signal enhancement can be obtained with high sensitivity for the trace concentrations as low as ppm level. It is concluded that surface-enhanced Raman spectroscopy can provide a fast, direct and precise detecting method for the detection of trace degradation solution from horns.
采用微波法在柠檬酸钠溶液和硝酸银溶液的混合物中合成了纳米银胶体。该方法具有加热速度快、温度分布均匀、反应条件易于控制等优点。通过准弹性激光散射（QLS）对银颗粒的尺寸和尺寸分布进行了表征。银颗粒的平均粒径为（53.27±2.65）nm，粒径主要分布在56nm左右。以银胶体为活性基底，获得了羊角降解产物的表面增强拉曼光谱。结果表明，与常规拉曼光谱相比，表面增强拉曼光谱的拉曼信号得到了显著增强，尤其是在反映降解产物中生化成分的659、830、850、929、999、1028、1280、1439和1599cm-1拉曼波段。对羊角降解产物的特征拉曼谱带进行了初步归属。归属结果表明，羊角降解产物的主要成分是氨基酸和多肽。这是首次将表面增强拉曼光谱用于检测羊角中的微量降解产物。对于低至ppm级的痕量浓度，表面增强拉曼光谱能够获得高灵敏度的信号增强。结论是，表面增强拉曼光谱可为检测羊角痕量降解溶液提供一种快速、直接和精确的检测方法。
