Key Laboratory for Renewable Energy and Gas Hydrates, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2013 May;108:177-80. doi: 10.1016/j.saa.2013.01.098. Epub 2013 Feb 15.
The nano-silver film was prepared by electrolysis method. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were employed to detect the morphology of the nano-silver particles. The SERS spectra of the hemoglobin (rabbit and mouse) on nano-silver film were gained. It could be known from the SERS spectra that the nano-silver films could enhance the Raman signal of the hemoglobin efficiently, and the sodium citrate and PBS create no influence to the SERS spectra of the hemoglobin. Using this electrolysis technique to fabricate highly bio-active, stable, reusable, and low-cost SERS substrate will be useful in the development of hemoglobin detection.
纳米银膜采用电解法制备。利用透射电子显微镜（TEM）和扫描电子显微镜（SEM）检测纳米银颗粒的形态。获得了纳米银膜上血红蛋白（兔和鼠）的表面增强拉曼散射（SERS）光谱。从 SERS 光谱中可以看出，纳米银膜可以有效地增强血红蛋白的拉曼信号，而柠檬酸钠和 PBS 对血红蛋白的 SERS 光谱没有影响。使用这种电解技术制备高生物活性、稳定、可重复使用且低成本的 SERS 基底，将有助于血红蛋白检测的发展。
