用于分离人双歧杆菌的选择性培养基
[Selective medium to isolate human Bifidobacterium].
作者信息
Huo Dongxue, Zhang Jiachao, Bai Na, Huang Weiqiang, Zhang Heping
出版信息
Wei Sheng Wu Xue Bao. 2014 Apr 4;54(4):433-41.
OBJECTIVE
To compare five selective media to isolate human Bifidobacterium.
METHODS
Feces from six healthy human volunteers were diluted and cultivated on five Bifidobacterium selective media. After anaerobic cultivation, bacterial colonies were counted, selected and identified. Meanwhile, bacterial genomic DNA was extracted from the feces samples, and the Denaturing Gradient Gel Electrophoresis (DGGE) and Quantitative Polymerase Chain Reaction (q-PCR) were applied to reveal the diversity of Bifidobacterium.
RESULTS
The amount of Bifidobacterium grown on BSM and BLM media was similar to the result detected by q-PCR and was significantly higher than that on three other media. Bifidobacterium isolated from BLM medium was similar to the identified result of DGGE profile.
CONCLUSION
BLM medium is the best selective medium for Bifidobacterium isolation from human gastrointestinal tract.
目的
比较五种用于分离人双歧杆菌的选择性培养基。
方法
将六名健康人类志愿者的粪便进行稀释，并在五种双歧杆菌选择性培养基上培养。厌氧培养后，对细菌菌落进行计数、挑选和鉴定。同时，从粪便样本中提取细菌基因组DNA，并应用变性梯度凝胶电泳（DGGE）和定量聚合酶链反应（q-PCR）来揭示双歧杆菌的多样性。
结果
在BSM和BLM培养基上生长的双歧杆菌数量与q-PCR检测结果相似，且显著高于其他三种培养基上的数量。从BLM培养基分离出的双歧杆菌与DGGE图谱的鉴定结果相似。
结论
BLM培养基是从人胃肠道分离双歧杆菌的最佳选择性培养基。
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