[Selective medium to isolate human Bifidobacterium].

OBJECTIVE

To compare five selective media to isolate human Bifidobacterium.

METHODS

Feces from six healthy human volunteers were diluted and cultivated on five Bifidobacterium selective media. After anaerobic cultivation, bacterial colonies were counted, selected and identified. Meanwhile, bacterial genomic DNA was extracted from the feces samples, and the Denaturing Gradient Gel Electrophoresis (DGGE) and Quantitative Polymerase Chain Reaction (q-PCR) were applied to reveal the diversity of Bifidobacterium.

RESULTS

The amount of Bifidobacterium grown on BSM and BLM media was similar to the result detected by q-PCR and was significantly higher than that on three other media. Bifidobacterium isolated from BLM medium was similar to the identified result of DGGE profile.

CONCLUSION

BLM medium is the best selective medium for Bifidobacterium isolation from human gastrointestinal tract.