利用棕榈油农业精炼废物生产和表征鼠李糖脂。
Production and characterization of rhamnolipid using palm oil agricultural refinery waste.
机构信息
School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester, United Kingdom; Department of Biological and Agricultural Engineering, Faculty of Engineering, Universiti Putra Malaysia, Malaysia.
School of Biomedical Sciences, Faculty of Life and Health Sciences, University of Ulster, Northern Ireland, United Kingdom.
出版信息
Bioresour Technol. 2017 Feb;225:99-105. doi: 10.1016/j.biortech.2016.11.052. Epub 2016 Nov 16.
In this research we assess the feasibility of using palm oil agricultural refinery waste as a carbon source for the production of rhamnolipid biosurfactant through fermentation. The production and characterization of rhamnolipid produced by Pseudomonas aeruginosa PAO1 grown on palm fatty acid distillate (PFAD) under batch fermentation were investigated. Results show that P. aeruginosa PAO1 can grow and produce 0.43gL of rhamnolipid using PFAD as the sole carbon source. Identification of the biosurfactant product using mass spectrometry confirmed the presence of monorhamnolipid and dirhamnolipid. The rhamnolipid produced from PFAD were able to reduce surface tension to 29mNm with a critical micelle concentration (CMC) 420mgL and emulsify kerosene and sunflower oil, with an emulsion index up to 30%. Results demonstrate that PFAD could be used as a low-cost substrate for rhamnolipid production, utilizing and transforming it into a value added biosurfactant product.
在这项研究中，我们评估了利用棕榈油农业精炼厂废物作为碳源通过发酵生产鼠李糖脂生物表面活性剂的可行性。研究了在分批发酵中，利用棕榈酸甲酯（PFAD）作为唯一碳源培养的铜绿假单胞菌 PAO1 生产鼠李糖脂的生产和特性。结果表明，铜绿假单胞菌 PAO1 可以利用 PFAD 生长并产生 0.43gL 的鼠李糖脂。使用质谱鉴定生物表面活性剂产物证实了单鼠李糖脂和双鼠李糖脂的存在。从 PFAD 生产的鼠李糖脂能够将表面张力降低到 29mNm，临界胶束浓度（CMC）为 420mgL，并乳化煤油和葵花籽油，乳化指数高达 30%。结果表明，PFAD 可以用作生产鼠李糖脂的低成本基质，将其利用并转化为具有附加值的生物表面活性剂产品。
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