Qian Gang, Ping Junjiao, Lu Jian, Zhang Zhen, Wang Lei, Xu Delin
Department of Cell Biology and Genetics, Zunyi Medical College, 201 Dalian Road, Zunyi, 563003, Guizhou, China,
Biochem Genet. 2014 Dec;52(11-12):494-508. doi: 10.1007/s10528-014-9662-9. Epub 2014 Jul 10.
Senecio scandens Buch.-Ham. ex D. Don (Compositae) is a crucial source of Chinese traditional medicine with antibacterial properties. We constructed a cDNA library and obtained expressed sequence tags (ESTs) to show the distribution of gene ontology annotations for mRNAs, using an individual plant with superior antibacterial characteristics. Analysis of comparative genomics indicates that the putative uncharacterized proteins (21.07%) might be derived from "molecular function unknown" clones or rare transcripts. Furthermore, the Compositae had high cross-species transferability of EST-derived simple sequence repeats (EST-SSR), based on valid amplifications of 206 primer pairs developed from the newly assembled expressed sequence tag sequences in Artemisia annua L. Among those EST-SSR markers, 52 primers showed polymorphic amplifications between individuals with contrasting diverse antibacterial traits. Our sequence data and molecular markers will be cost-effective tools for further studies such as genome annotation, molecular breeding, and novel transcript profiles within Compositae species.
千里光（菊科千里光属布赫 - 汉密尔顿氏植物，由D. 唐命名）是具有抗菌特性的重要中药来源。我们构建了一个cDNA文库，并使用一株具有卓越抗菌特性的单株植物获得了表达序列标签（EST），以展示mRNA的基因本体注释分布。比较基因组学分析表明，推定的未表征蛋白质（21.07%）可能源自“分子功能未知”的克隆或稀有转录本。此外，基于从新组装的青蒿表达序列标签序列开发的206对引物的有效扩增，菊科植物的EST衍生简单序列重复（EST - SSR）具有较高的跨物种转移性。在这些EST - SSR标记中，52对引物在具有不同抗菌性状对比的个体之间显示出多态性扩增。我们的序列数据和分子标记将成为菊科物种进一步研究（如基因组注释、分子育种和新转录本概况）的经济高效工具。
