Qian Gang, Ping Junjiao, Zhang Zhen, Xu Delin
Department of Cell Biology and Genetics, Zunyi Medical College , Zunyi , Guizhou , P.R. China.
Biotechnol Biotechnol Equip. 2014 Sep 3;28(5):805-812. doi: 10.1080/13102818.2014.956461. Epub 2014 Oct 28.
Buch.-Ham. ex D. Don, an important antibacterial source of Chinese traditional medicine, has a widespread distribution in a few ecological habitats of China. We generated a full-length complementary DNA (cDNA) library from a sample of elite individuals with superior antibacterial properties, with satisfactory parameters such as library storage (4.30 × 10 CFU), efficiency of titre (1.30 × 10 CFU/mL), transformation efficiency (96.35%), full-length ratio (64.00%) and redundancy ratio (3.28%). The BLASTN search revealed the facile formation of counterparts between the experimental sample and in view of high-homology cDNA sequence (90.79%) with -values <1 - 50. Sequence similarities to known proteins indicate that the entire sequences of the full-length cDNA clones consist of the major of functional genes identified by a large set of microarray data from the present experimental material. For other Compositae species, a large set of full-length cDNA clones reported in the present article will serve as a useful resource to facilitate further research on the transferability of expressed sequence tag-derived simple sequence repeats (EST-SSR) development, comparative genomics and novel transcript profiles.
地锦草（Buch.-Ham. ex D. Don）是中国传统医学中一种重要的抗菌药材，在中国的一些生态栖息地广泛分布。我们从具有卓越抗菌特性的优良个体样本中构建了一个全长互补DNA（cDNA）文库，该文库具有令人满意的参数，如图库库容（4.30×10 CFU）、滴度效率（1.30×10 CFU/mL）、转化效率（96.35%）、全长比例（64.00%）和冗余比例（3.28%）。BLASTN搜索显示，鉴于实验样本与具有<1 - 50的 - 值的高同源性cDNA序列（90.79%）之间易于形成对应物。与已知蛋白质的序列相似性表明，全长cDNA克隆的整个序列由来自本实验材料的大量微阵列数据鉴定的主要功能基因组成。对于其他菊科物种，本文报道的大量全长cDNA克隆将作为一种有用的资源，以促进对表达序列标签衍生的简单序列重复（EST-SSR）开发、比较基因组学和新转录本谱的可转移性的进一步研究。
