Ou LiJuan, Li XiaoYan, Liu HongWei, Li LiJuan, Chu Xia
College of Material and Chemical Engineering, Hunan Institute of Technology.
Anal Sci. 2014;30(7):723-7. doi: 10.2116/analsci.30.723.
Polythymine (poly T)-templated copper nanoparticles (CuNPs) were demonstrated as novel and sensitive fluorescence probes for the detection of Pb(2+) based on the fluorescence quenching effect. The as-prepared CuNPs displayed strong fluorescence emission. However, the fluorescence of CuNPs was readily quenched in the presence of Pb(2+). These changes in fluorescence intensity of CuNPs allowed for the analysis of Pb(2+) with rapidity (<10 min), simplicity (label-free), high sensitivity (LOD 0.4 nM), high selectivity (no interference from other metal ions) and at low-cost (without any labels and sophisticated operation). We validated the practicality of using CuNPs for the determination of Pb(2+) in environmental samples through analyses of tap water samples.
基于荧光猝灭效应，聚胸腺嘧啶（poly T）模板化的铜纳米颗粒（CuNPs）被证明是用于检测Pb(2+)的新型灵敏荧光探针。所制备的CuNPs表现出强烈的荧光发射。然而，在Pb(2+)存在的情况下，CuNPs的荧光很容易被猝灭。CuNPs荧光强度的这些变化使得能够快速（<10分钟）、简单（无需标记）、高灵敏度（检测限0.4 nM）、高选择性（不受其他金属离子干扰）且低成本（无需任何标记和复杂操作）地分析Pb(2+)。我们通过对自来水样品的分析验证了使用CuNPs测定环境样品中Pb(2+)的实用性。
