Pfüller U, Ichev K, Ovtscharoff W, Franz H
Staatliches Institut für Immunpräparate und Nährmedien, Berlin, GDR.
Acta Histochem. 1989;85(2):203-11. doi: 10.1016/S0065-1281(89)80069-8.
17 Polyethyleneimine-metal complexes were synthesized and 3 of them were tested cytochemically for visualization of negative tissue charges. The demonstration of the anionic sites was carried out on rat cerebral cortex and on frog cutaneous pectoral muscle. As controls, neuraminidase digestion, methylation, and omission of osmium-postfixation were used. The osmiophilic properties of polyethyleneimine, polyethyleneimine salts, and polyethyleneimine-metal complexes were discussed.
合成了17种聚乙烯亚胺 - 金属络合物，并对其中3种进行了细胞化学测试，以观察阴性组织电荷。在大鼠大脑皮层和青蛙胸皮肌上进行了阴离子位点的显示。作为对照，使用了神经氨酸酶消化、甲基化和省略锇后固定。讨论了聚乙烯亚胺、聚乙烯亚胺盐和聚乙烯亚胺 - 金属络合物的嗜锇特性。
