Ichev K, Ovtscharoff W, Pfüller U, Franz H
Histochemistry. 1984;81(5):501-4. doi: 10.1007/BF00489758.
Electron-dense material in clear synaptic vesicles in rat cerebral cortex and neuromuscular junctions of frog cutaneous pectoris muscle was demonstrated by using ferrocenyl cationics. Electron-dense spots were usually attached to the inner surface of the vesicular membrane. Control experiments (treatment with Triton X-100 or cetylpyridinium chloride; enzyme digestion with trypsin, hyaluronidase, neuraminidase, sulfatase and beta-glucuronidase) suggested that the electron-dense material is a glycoprotein.
通过使用二茂铁基阳离子试剂，在大鼠大脑皮层的清亮突触小泡以及青蛙胸大肌的神经肌肉接头处发现了电子致密物质。电子致密斑点通常附着于小泡膜的内表面。对照实验（用曲拉通X-100或十六烷基氯化吡啶处理；用胰蛋白酶、透明质酸酶、神经氨酸酶、硫酸酯酶和β-葡萄糖醛酸酶进行酶消化）表明，该电子致密物质是一种糖蛋白。
