硬脂酰辅酶A去饱和酶抑制作用可阻断丙型肝炎病毒诱导的特殊膜的形成。

Stearoyl-CoA desaturase inhibition blocks formation of hepatitis C virus-induced specialized membranes.

作者信息

Lyn Rodney K, Singaravelu Ragunath, Kargman Stacia, O'Hara Shifawn, Chan Helen, Oballa Renata, Huang Zheng, Jones Daniel M, Ridsdale Andrew, Russell Rodney S, Partridge Anthony W, Pezacki John Paul

机构信息

1] National Research Council of Canada, Ottawa, Ontario, Canada [2] Department of Chemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada.

1] National Research Council of Canada, Ottawa, Ontario, Canada [2] Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada.

出版信息

Sci Rep. 2014 Apr 1;4:4549. doi: 10.1038/srep04549.

Hepatitis C virus (HCV) replication is dependent on the formation of specialized membrane structures; however, the host factor requirements for the formation of these HCV complexes remain unclear. Herein, we demonstrate that inhibition of stearoyl-CoA desaturase 1 (SCD-1) halts the biosynthesis of unsaturated fatty acids, such as oleic acid, and negatively modulates HCV replication. Unsaturated fatty acids play key roles in membrane curvature and fluidity. Mechanistically, we demonstrate that SCD-1 inhibition disrupts the integrity of membranous HCV replication complexes and renders HCV RNA susceptible to nuclease-mediated degradation. Our work establishes a novel function for unsaturated fatty acids in HCV replication.