Besaury Ludovic, Pawlak Barbara, Quillet Laurent
Laboratoire de Microbiologie Signaux et Microenvironnement, EA 4312, Bâtiment IRESE B, 2ème étage, UFR des Sciences, Université de Rouen, 76821, Mont Saint Aignan, France.
Environ Sci Pollut Res Int. 2016 Mar;23(5):4013-23. doi: 10.1007/s11356-014-3254-4. Epub 2014 Jul 11.
Microorganisms have developed copper-resistance mechanisms in order to survive in contaminated environments. The abundance and expression of the copper-resistance genes cusA and copA, encoding respectively for a Resistance Cell Nodulation protein and for a P-type ATP-ase pump, was assessed along a gradient of copper concentration in microcosms prepared from Seine estuary mudflat sediment. We demonstrated that the abundance of copA and cusA genes decreased with the increase of copper concentration and that cusA gene was up to ten times higher than the copA gene. Only the copA gene was expressed in both oxic and anoxic conditions. The abundance and activity of the microbial community remained constant whatever the concentrations of copper along the gradient. The molecular phylogeny of the two copper-resistance genes was studied and revealed that the increase of copper increased the diversity of copA and cusA gene sequences.
微生物已经进化出抗铜机制以便在受污染环境中生存。分别编码一种抗药细胞结瘤蛋白和一种P型ATP酶泵的抗铜基因cusA和copA的丰度及表达，是在由塞纳河河口泥滩沉积物制备的微观世界中沿着铜浓度梯度进行评估的。我们证明，copA和cusA基因的丰度随着铜浓度的增加而降低，并且cusA基因比copA基因高多达十倍。只有copA基因在有氧和无氧条件下均有表达。无论沿着梯度的铜浓度如何，微生物群落的丰度和活性都保持恒定。对这两个抗铜基因的分子系统发育进行了研究，结果表明铜含量的增加增加了copA和cusA基因序列的多样性。
