UMR CNRS 6143 M2C-groupe Microbiologie, Université de Rouen, Mont Saint Aignan, Rouen, France.
Microb Ecol. 2013 Feb;65(2):311-24. doi: 10.1007/s00248-012-0120-0. Epub 2012 Sep 14.
Cultivation and molecular-based approaches were used to study microbial diversity in two Chilean marine sediments contaminated with high (835 ppm) and very high concentrations of copper (1,533 ppm). The diversity of cultivable bacteria resistant to copper was studied at oxic and anoxic conditions, focusing on sulfate-, thiosulfate-, and iron-reducing bacteria. For both sediments, the cultivable bacteria isolated at oxic conditions were mostly affiliated to the genus Bacillus, while at anoxic conditions the majority of the cultivable bacteria found were closely related to members of the genera Desulfovibrio, Sphingomonas, and Virgibacillus. Copper resistance was between 100 and 400 ppm, with the exception of a strain affiliated to members of the genus Desulfuromonas, which was resistant up to 1,000 ppm of copper. In parallel, cloning and sequencing of 16S rRNA was performed to study the total bacterial diversity in the sediments. A weak correlation was observed between the isolated strains and the 16S rRNA operational taxonomic units detected. The presence of copper resistance genes (copA, cusA, and pcoA) was tested for all the strains isolated; only copA was detected in a few isolates, suggesting that other copper resistance mechanisms could be used by the bacteria in those highly copper-contaminated sediments.
采用培养和基于分子的方法研究了智利两个受高浓度（835ppm）和极高浓度铜（1533ppm）污染的海洋沉积物中的微生物多样性。在好氧和缺氧条件下研究了对铜具有抗性的可培养细菌的多样性，重点研究了硫酸盐、硫代硫酸盐和铁还原细菌。对于这两种沉积物，在好氧条件下分离的可培养细菌主要与芽孢杆菌属有关，而在缺氧条件下发现的大多数可培养细菌与脱硫弧菌属、鞘氨醇单胞菌属和弧菌属的成员密切相关。铜抗性在 100 至 400ppm 之间，只有一株与脱硫单胞菌属的菌株对高达 1000ppm 的铜具有抗性。同时，还进行了 16S rRNA 的克隆和测序，以研究沉积物中的总细菌多样性。观察到分离株与检测到的 16S rRNA 操作分类单位之间存在弱相关性。对所有分离的菌株都进行了铜抗性基因（copA、cusA 和 pcoA）的检测；仅在少数分离株中检测到 copA，表明在这些高铜污染的沉积物中，细菌可能使用其他铜抗性机制。
