2型糖尿病患者外周血中三个基因（CAMK1D、CRY2和CALM2）启动子DNA甲基化的研究
Investigation into the promoter DNA methylation of three genes (CAMK1D, CRY2 and CALM2) in the peripheral blood of patients with type 2 diabetes.
作者信息
Cheng Jia, Tang Linlin, Hong Qingxiao, Ye Huadan, Xu Xuting, Xu Leiting, Bu Shizhong, Wang Qinwen, Dai Dongjun, Jiang Danjie, Duan Shiwei
机构信息
Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China ; Department of Clinical Medicine, Ningbo Kangning Hospital, Ningbo, Zhejiang 315201, P.R. China.
Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China.
出版信息
Exp Ther Med. 2014 Aug;8(2):579-584. doi: 10.3892/etm.2014.1766. Epub 2014 Jun 6.
Promoter DNA methylation may reflect the interaction between genetic backgrounds and environmental factors in the development of metabolic disorders, including type 2 diabetes (T2D). Calcium/calmodulin-dependent protein kinase 1D (CAMK1D), cryptochrome 2 (CRY2) and calmodulin 2 (CALM2) genes have been identified to be associated with a risk of T2D. Therefore, the aim of the present study was to investigate the contribution of promoter DNA methylation of these genes to the risk of T2D. Using bisulfite pyrosequencing technology, the DNA methylation levels of the CpG dinucleotides within the CAMK1D, CRY2 and CALM2 gene promoters were measured in 48 patients with T2D and 48 age- and gender-matched healthy controls. The results demonstrated that the promoters of these three genes were hypomethylated in the peripheral blood of all the subjects, and DNA methylation of these three genes did not contribute to the risk of T2D.
启动子DNA甲基化可能反映了代谢紊乱（包括2型糖尿病，T2D）发生发展过程中遗传背景与环境因素之间的相互作用。钙/钙调蛋白依赖性蛋白激酶1D（CAMK1D）、隐花色素2（CRY2）和钙调蛋白2（CALM2）基因已被确定与T2D风险相关。因此，本研究旨在探讨这些基因的启动子DNA甲基化对T2D风险的影响。采用亚硫酸氢盐焦磷酸测序技术，对48例T2D患者和48例年龄及性别匹配的健康对照者的CAMK1D、CRY2和CALM2基因启动子内的CpG二核苷酸的DNA甲基化水平进行了检测。结果表明，这三个基因的启动子在所有受试者的外周血中均呈低甲基化状态，且这三个基因的DNA甲基化与T2D风险无关。
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