Subramanian Palaniappan, Mazurenko Ievgen, Zaitsev Vladimir, Coffinier Yannick, Boukherroub Rabah, Szunerits Sabine
Institut de Recherche Interdisciplinaire (IRI), CNRS USR 3078, Université Lille1, Parc de la Haute Borne, 50 avenue de Halley, BP 70478, 59658 Villeneuve d'Ascq, France.
Analyst. 2014 Sep 7;139(17):4343-9. doi: 10.1039/c4an00146j.
Coating boron-doped diamond nanowires (BDD NWs) with a conducting polymer, poly[3-(pyrrolyl)carboxylic acid], has been reported. Polymer coating was achieved through electropolymerization of 3-(pyrrolyl)carboxylic acid at the electrode interface by amperometrically biasing the BDD NWs interface until a predefined charge has passed. The poly[3-(pyrrolyl)carboxylic acid] modified BDD NWs (PPA-BDD NWs) were characterized by scanning electron microscopy (SEM) and cyclic voltammetry (CV). Using a deposition charge of 11 mC cm(-2) resulted in a thin polymer film deposition. The availability of the carboxylic groups of the polymer coated BDD NWs electrode was demonstrated through copper ion (Cu(2+)) chelation. The resulting complex was successfully used for the site-specific immobilization of histidine-tagged peptides. The binding process was followed by electrochemical impedance spectroscopy (EIS). The Cu(2+)-chelated PPA-BDD NWs interface showed peptide loading capability comparable to those of commercially available interfaces and can be easily regenerated several times using ethylenediaminetetraacetic acid (EDTA).
据报道，已将导电聚合物聚[3-(吡咯基)羧酸]涂覆在掺硼金刚石纳米线（BDD NWs）上。通过在电极界面上对3-(吡咯基)羧酸进行电聚合来实现聚合物涂层，方法是对BDD NWs界面进行安培偏置，直到通过预定义的电荷量。通过扫描电子显微镜（SEM）和循环伏安法（CV）对聚[3-(吡咯基)羧酸]修饰的BDD NWs（PPA-BDD NWs）进行了表征。使用11 mC cm(-2)的沉积电荷量可得到薄聚合物膜沉积。通过铜离子（Cu(2+)）螯合证明了聚合物涂覆的BDD NWs电极中羧基的可用性。所得配合物成功用于组氨酸标签肽的位点特异性固定。通过电化学阻抗谱（EIS）跟踪结合过程。Cu(2+)螯合的PPA-BDD NWs界面显示出与市售界面相当的肽负载能力，并且可以使用乙二胺四乙酸（EDTA）轻松再生几次。
