Rocha R M P, Alves A M C V, Lima L F, Duarte A B G, Chaves R N, Brito I R, Costa E C, Bernuci M P, Rosa-e-Silva A C J S, Xu M, Rodrigues A P R, Campello C C, Figueiredo J R
Faculty of Veterinary Medicine, LAMOFOPA, State University of Ceara, Fortaleza, Ceara, Brazil.
Faculty of Veterinary Medicine, LAMOFOPA, State University of Ceara, Fortaleza, Ceara, Brazil.
Domest Anim Endocrinol. 2014 Oct;49:27-30. doi: 10.1016/j.domaniend.2014.04.005. Epub 2014 May 9.
The present study evaluated the efficiency of using 2 culture media developed for mice and for goats in the in vitro preantral follicle culture of each species. Murine and caprine secondary follicles were cultured in vitro with human recombinant follicle-stimulating hormone (murine medium) or with bovine recombinant follicle-stimulating hormone in association with growth hormone (caprine medium). The results showed that murine follicles cultured in caprine medium had lower (P < 0.05) rates of follicular survival and growth, whereas for caprine follicles, these variables were not affected by the type of medium used (P > 0.05). After in vitro maturation, a higher (P < 0.05) number of oocytes that resumed meiosis were observed in the murine medium for both species. In contrast, only in the caprine species estradiol production was significantly superior when the caprine medium was used. Higher progesterone production was observed in the presence of the murine medium only for murine follicles (P < 0.05). In conclusion, murine and caprine preantral follicles cultured under the same in vitro culture medium conditions respond differently; caprine oocytes grown in vitro in the presence of the murine medium show the greatest developmental competence among the tested combinations. Therefore, under the present experimental conditions, the mouse follicle culture has proved be a good model for the development of new culture media for caprine preantral follicles.
本研究评估了两种分别为小鼠和山羊研发的培养基在体外培养各物种腔前卵泡时的效率。将小鼠和山羊的次级卵泡分别在体外用人重组促卵泡激素（小鼠培养基）或牛重组促卵泡激素与生长激素联合使用（山羊培养基）进行培养。结果显示，在山羊培养基中培养的小鼠卵泡，其卵泡存活和生长率较低（P < 0.05），而对于山羊卵泡，这些变量不受所用培养基类型的影响（P > 0.05）。体外成熟后，在两种物种的小鼠培养基中观察到恢复减数分裂的卵母细胞数量更多（P < 0.05）。相反，仅在山羊物种中，使用山羊培养基时雌二醇产量显著更高。仅在小鼠卵泡中，在小鼠培养基存在的情况下观察到更高的孕酮产量（P < 0.05）。总之，在相同的体外培养基条件下培养的小鼠和山羊腔前卵泡反应不同；在小鼠培养基存在下体外生长的山羊卵母细胞在测试组合中显示出最大的发育能力。因此，在当前实验条件下，小鼠卵泡培养已被证明是开发山羊腔前卵泡新型培养基的良好模型。
