de Figueiredo José Ricardo, Cadenas Jesús, de Lima Laritza Ferreira, Santos Regiane Rodrigues
Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary, State University of Ceara, Fortaleza CE, Brazil.
Schothorst Feed Research, Lelystad, The Netherlands.
Anim Reprod. 2020 May 22;16(1):52-65. doi: 10.21451/1984-3143-AR2018-123.
The follicle culture (IVFC) represents an outstanding tool to enhance our understanding of the control of folliculogenesis and to allow the future use of a large number of immature oocytes enclosed in preantral follicles (PFs) in assisted reproductive techniques in humans as well as in others mammalian species including the ruminants. So far, the best results of IVFC were reported from mice with the production of live offspring from primordial follicles cultured in vitro. Live birth has been obtained after the in vitro culture of bovine early antral follicles. However, in other ruminant species, these results have been limited to the production of a variable number of mature oocytes and low percentages of embryos after in vitro culture of goat, buffalo and sheep isolated secondary preantral follicles. The present review presents and discusses the main findings, limitations, and prospects of in vitro folliculogenesis in ruminants focusing on bovine, caprine, and ovine species.
卵泡培养（IVFC）是一种杰出的工具，有助于我们深入了解卵泡发生的调控机制，并使未来能够在人类以及包括反刍动物在内的其他哺乳动物的辅助生殖技术中，利用大量封闭在腔前卵泡（PFs）中的未成熟卵母细胞。到目前为止，IVFC的最佳结果来自小鼠，体外培养的原始卵泡产生了活后代。牛早期腔卵泡体外培养后已获得活产。然而，在其他反刍动物物种中，这些结果仅限于山羊、水牛和绵羊分离的次级腔前卵泡体外培养后产生数量不等的成熟卵母细胞和低百分比的胚胎。本综述介绍并讨论了反刍动物体外卵泡发生的主要发现、局限性和前景，重点关注牛、山羊和绵羊物种。
