Chen Lin, Li Bo, Zhou Lizhi, Zhao Guanghong
a School of Resources and Environmental Engineering, Anhui University , Hefei , P. R. China and.
b Anhui Biodiversity Information Center , Hefei , P. R. China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):1119-20. doi: 10.3109/19401736.2014.933328. Epub 2014 Jul 10.
Predatory carp Chanodichthys erythropterus is a small-sized economic cyprinid fish distributed in East Asia. We sequenced its complete mitochondrial genome by PCR-based method. The mitochondrial DNA is packaged in a compact 16,629 base pair (bp) circular molecule with A + T content of 56.1%. It contains 22 transfer RNA genes, 13 protein-coding genes (PCGs), 2 ribosomal RNA genes, and the non-coding control region (D-loop). All PCGs are initiated by ATG codons, except for COI, which uses GTG as its start codon. Of the 13 PCGs, 12 stop with TAA and TAG, while Cyt b uses incomplete termination codon T. All tRNAs possess the classic cloverleaf secondary structure except for tRNA(Ser)(AGN), which lacks the ''DHU'' stem.
赤眼鳟是一种分布于东亚的小型经济鲤科鱼类。我们采用基于聚合酶链式反应（PCR）的方法对其线粒体全基因组进行了测序。线粒体DNA包装成一个紧凑的16629碱基对（bp）环状分子，A+T含量为56.1%。它包含22个转运RNA基因、13个蛋白质编码基因（PCGs）、2个核糖体RNA基因和非编码控制区（D-loop）。除细胞色素氧化酶亚基I（COI）使用GTG作为起始密码子外，所有PCGs均由ATG密码子起始。在13个PCGs中，12个以TAA和TAG作为终止密码子，而细胞色素b（Cyt b）使用不完全终止密码子T。除了缺乏“DHU”茎的丝氨酸转运RNA（tRNA(Ser)(AGN)）外，所有tRNA都具有经典的三叶草二级结构。
