Filczak K, Korbecki M
Acta Virol. 1978 Jan;22(1):70-3.
Polyethylene glycol (PEG) was used for precipitation of rubella virus haemagglutinating (HA) antigen from the fluid phase of infected Vero cell cultures, maintained with or without calf serum. In both cases potent antigen preparation were obtained. When the antigen was precipitated from serum-containing medium, higher titres were obtained, provided that substrate or final product were treated with Tween 80 and ether. HA antigen obtained by PET precipitation was entirely valuable in the haemagglutination-inhibition test, as compared with antigens prepared by other methods.
聚乙二醇（PEG）用于从感染风疹病毒的非洲绿猴肾细胞（Vero细胞）培养物的液相中沉淀血凝（HA）抗原，培养过程中添加或不添加小牛血清。在这两种情况下均获得了高效价的抗原制剂。当从含血清的培养基中沉淀抗原时，若底物或最终产物用吐温80和乙醚处理，则可获得更高的效价。与通过其他方法制备的抗原相比，通过PEG沉淀获得的HA抗原在血凝抑制试验中具有完全的价值。
