聚乙二醇处理的小牛血清在病毒和干扰素研究的细胞培养中的应用。
Use of polyethylene glycol-treated calf serum for cell cultures in virus and interferon studies.
作者信息
Inglot A D, Godzińska H, Chudzio T
出版信息
Acta Virol. 1975 May;19(3):250-4.
PMID:50724
Abstract
Immunoglobulins and lipoproteins can be efficiently removed from calf serum by precipitation with polyethylene glycol (PEG) 6000 under sterile conditions. The PEG-treated serum is suitable for cell cultures used for virus growth and assays. Moreover, PEG was found to slow down the growth of L cells and to enhance the production and activity of mouse interferon.
摘要
在无菌条件下，通过用聚乙二醇（PEG）6000沉淀，可以有效地从小牛血清中去除免疫球蛋白和脂蛋白。经PEG处理的血清适用于用于病毒生长和检测的细胞培养。此外，发现PEG可减缓L细胞的生长，并增强小鼠干扰素的产生和活性。
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