Sirén M, Portin P
Hereditas. 1989;110(2):175-8. doi: 10.1111/j.1601-5223.1989.tb00439.x.
Gene dosage studies of a temperature sensitive Abruptex mutation of the Notch locus of Drosophila melanogaster revealed, firstly, that hemizygous females were lethal at all temperatures studied, while hemizygous males were subvital at 18 degrees C, semilethal at 25 degrees C, and lethal at 29 degrees C. This is due to dosage compensation of the Notch locus. Secondly, it was observed that in general an increasing dose of the wild allele decreased the expression of Abruptex at all temperatures. An exception was the Axtsl/Axtsl/ + genotype, which was more expressed than Axtsl/Axtsl at low temperatures. This suggests antimorphism as the most likely mode of action of the temperature sensitive Abruptex mutation. Thirdly, it was observed that the expression of Abruptex phenotype increased from 18 degrees C to 25 degrees C but decreased from 25 degrees C to 29 degrees C if the wild type allele was involved in the genotype. This suggests that the gene product of the temperature sensitive Abruptex allele loses its function at high temperatures. The putative antimorphic mode of action of the temperature sensitive Abruptex mutation suggests that the Notch gene product interacts with products of other neurogenic loci or with itself.
对果蝇Notch基因座的一个温度敏感型Abruptex突变进行的基因剂量研究表明，首先，在所研究的所有温度下，半合子雌性均致死，而半合子雄性在18摄氏度时活力不足，在25摄氏度时半致死，在29摄氏度时致死。这是由于Notch基因座的剂量补偿作用。其次，观察到一般情况下，野生等位基因剂量的增加会在所有温度下降低Abruptex的表达。一个例外是Axtsl/Axtsl / +基因型，在低温下其表达量比Axtsl/Axtsl更高。这表明反形态作用是温度敏感型Abruptex突变最可能的作用模式。第三，观察到如果野生型等位基因参与基因型，Abruptex表型的表达从18摄氏度增加到25摄氏度，但从25摄氏度降低到29摄氏度。这表明温度敏感型Abruptex等位基因的基因产物在高温下失去其功能。温度敏感型Abruptex突变的假定反形态作用模式表明，Notch基因产物与其他神经发生基因座的产物相互作用或与自身相互作用。
